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Volume 20, Number 10—October 2014
Dispatch

Rickettsia parkeri and Rickettsia montanensis, Kentucky and Tennessee, USA

Benedict B. PagacComments to Author , Melissa K. Miller, Meagan C. Mazzei, David H. Nielsen, Ju Jiang, and Allen L. Richards
Author affiliations: US Army Public Health Command Region North, Fort George G. Meade, Maryland, USA (B.B. Pagac, M.K. Miller, M.C. Mazzei, D.H. Nielsen); Naval Medical Research Center, Silver Spring, Maryland, USA (J. Jiang, A.L. Richards)

Main Article

Table 2

Primers used for PCR, nested PCR, and sequencing for Rickettsia parkeri and Rickettsia montanensis, Kentucky and Tennessee, USA, 2012*

Gene, primer Sequence (5′→3′) Fragment, bp
ompB
120-M59 CCGCAGGGTTGGTAACTGC
ompB1570R TCGCCGGTAATTRTAGCACT PCR: 1,540
120–607F AATATCGCTGACGGTCAAGGT
120–807R CCTTTTAGATTACCGCCTAA
ompA
190–3588F AACAGTGAATGTAGGAGCAG
RompA3182R TTGCTGAGCGAAAYACTTACTYC PCR: 3,202
190–5238R ACTATTAAAGGCTAGGCTATT Nested PCR: 1,651
RhoA4336F AGTTCAGGAAACGACCGTA
RompA4433R TTTCCTGCAGTTACAGAATTTAAT

*omp, outer membrane protein.

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Page created: September 22, 2014
Page updated: September 22, 2014
Page reviewed: September 22, 2014
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