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Volume 20, Number 10—October 2014
Letter

Marburgvirus Resurgence in Kitaka Mine Bat Population after Extermination Attempts, Uganda

Brian Amman, Luke Nyakarahuka, Anita K. McElroy, Kimberly A. Dodd, Tara Sealy, Amy J. Schuh, Trevor R. Shoemaker, Stephen Balinandi, Patrick Atimnedi, Winyi Kaboyo, Stuart T. Nichol, and Jonathan S. TownerComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (B.R. Amman, A.K. McElroy, K.A. Dodd, T.K. Sealy, A.J. Schuh, S.T. Nichol, J.S. Towner); Uganda Virus Research Institute, Entebbe, Uganda (L. Nyakarahuka); Emory University, Atlanta (A.K. McElroy); University of California, Davis, California, USA (K.A. Dodd); Centers for Disease Control and Prevention, Entebbe (T.R. Shoemaker, S. Balinandi); Uganda Wildlife Authority, Kampala, Uganda (P. Atimnedi); Uganda Ministry of Health, Kampala (W. Kaboyo)

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Figure

Phylogeny of concatenated marburgvirus nucleoprotein (NP) and viral protein 35 (VP35) gene fragments as determined by using the maximum-likelihood method. Sequences from the NP (289–372 nt) and VP35 (203–213 nt) genes were amplified and determined from viral RNA and then sequenced as described elsewhere (4). Sequence names shown in red font represent those generated from samples collected from bats during the November 2012 outbreak investigation at Kitaka Mine, Uganda. Sequence names in blue fon

Figure. Phylogeny of concatenated marburgvirus nucleoprotein (NP) and viral protein 35 (VP35) gene fragments as determined by using the maximum-likelihood method. Sequences from the NP (289–372 nt) and VP35 (203–213 nt) genes were amplified and determined from viral RNA and then sequenced as described elsewhere (4). Sequence names shown in red font represent those generated from samples collected from bats during the November 2012 outbreak investigation at Kitaka Mine, Uganda. Sequence names in blue font represent those generated from samples obtained from marburgvirus-infected persons in Kabale and Ibanda, Uganda, in 2012. Multiple sequence alignments were generated, and a maximum-likelihood analysis was conducted on concatenated NP and VP35 (208–580 nt) sequences by using the PhyML method in conjunction with the GTR+I+G nucleotide substitution model implemented in SeaView version 4.2.12 (10). NP and VP35 gene sequences determined from samples in this study (in red) were submitted to GenBank (accession nos. KJ747211–KJ747234 and KJ747235–KJ747253, respectively). Bayesian posterior probabilities above 50 are shown at the nodes. Scale bar indicates nucleotide substitutions per site. Ang, Angola; DRC, Democratic Republic of Congo; Gab, Gabon; Ger, Germany; Ken, Kenya; Net, Netherlands; Rav, Ravn virus; Uga, Uganda; Zim, Zimbabwe.

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References
  1. Adjemian  J, Farnon  EC, Tschioko  F, Wamala  JF, Byaruhanga  E, Bwire  GS, Outbreak of Marburg hemorrhagic fever among miners in Kamwenge and Ibanda Districts, Uganda, 2007. J Infect Dis. 2011;204(Suppl 3):S796–9 . DOIPubMedGoogle Scholar
  2. Timen  A, Koopmans  MP, Vossen  AC, van Doornum  GJ, Gunther  S, van den Berkmortel  F, Response to imported case of Marburg hemorrhagic fever, the Netherlands. Emerg Infect Dis. 2009;15:1171–5 . DOIPubMedGoogle Scholar
  3. Centers for Disease Control and Prevention. Imported case of Marburg hemorrhagic fever–Colorado, 2008. MMWR Morb Mortal Wkly Rep. 2009;58:1377–81 .PubMedGoogle Scholar
  4. Amman  BR, Carroll  SA, Reed  ZD, Sealy  TK, Balinandi  S, Swanepoel  R, Seasonal pulses of Marburg virus circulation in juvenile Rousettus aegyptiacus bats coincide with periods of increased risk of human infection. PLoS Pathog. 2012;8:e1002877 . DOIPubMedGoogle Scholar
  5. Towner  JS, Amman  BR, Sealy  TK, Carroll  SA, Comer  JA, Kemp  A, Isolation of genetically diverse Marburg viruses from Egyptian fruit bats. PLoS Pathog. 2009;5:e1000536 . DOIPubMedGoogle Scholar
  6. Donnelly  CA, Woodroffe  R, Cox  DR, Bourne  J, Gettinby  G, Le Fevre  AM, Impact of localized badger culling on tuberculosis incidence in British cattle. Nature. 2003;426:834–7. DOIPubMedGoogle Scholar
  7. Swanepoel  R. Rabies. In: Coetzer JAW, Tustin RC, editors. Infectious diseases of livestock. 2nd ed. Cape Town (South Africa): Oxford University Press Southern Africa; 2014. p. 1123–82.
  8. Albariño  CG, Shoemaker  T, Khristova  ML, Wamala  JF, Muyembe  JJ, Balinandi  S, Genomic analysis of filoviruses associated with four viral hemorrhagic fever outbreaks in Uganda and the Democratic Republic of the Congo in 2012. Virology. 2013;442:97–100. DOIPubMedGoogle Scholar
  9. Maganga  GD, Bourgarel  M, Ella  GE, Drexler  JF, Gonzalez  JP, Drosten  C, Is Marburg virus enzootic in Gabon? J Infect Dis. 2011;204(Suppl 3):S800–3 . DOIPubMedGoogle Scholar
  10. Gouy  M, Guindon  S, Gascuel  O. SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol. 2010;27:221–4. DOIPubMedGoogle Scholar

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Page updated: September 23, 2014
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