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Volume 20, Number 12—December 2014
Dispatch

Novel Bluetongue Virus in Goats, Corsica, France, 2014

Stéphan ZientaraComments to Author , Corinne Sailleau, Cyril Viarouge, Dirck Höper, Martin Beer, Maria Jenckel, Bernd Hoffmann, Aurore Romey, Labib Bakkali-Kassimi, Aurore Fablet, Damien Vitour, and Emmanuel Bréard
Author affiliations: ANSES (French Agency for Food, Environmental and Occupational Health and Safety); Maisons-Alfort, France (S. Zientara, C. Sailleau, C. Viarouge, A. Romey, L. Bakkali-Kassimi, A. Fablet, D. Vitour, E. Bréard); Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (D. Höper, M. Beer, M Jenckel, B. Hoffmann)

Main Article

Table

Primers and probe used to detect the BTV strain BTV-n in samples from goats, Corsica, France, 2014*

PCR type Target Probe and primer names Primer sequence, 5′ → 3′ Nucleotide location
Conventional BTV RT-PCR Segment 2 PF YRWTTGATTTTGARAARGA 1549–1566
PR
 GAAYCGACCACTGCCTATG
 2355–2337
Conventional RT-PCR BTV-n segment 2 BTV-n F CAGATCTGGTTTTACCGAG 1546–1564
BTV-n R
 ATGATCCATCGGACTTAACT
 1949–1927
Real-time RT-PCR BTV-n segment 2 BTV-n F3 TGGATCATGATGGTTATGAACACC 1942–1966
BTV-n R3 CGCCTCTCCAATCTCACGTATT 2102–2081
BTV-n FAM-TGACTATGCGAGGTTGG-MGB 1987–2004

*BTV, bluetongue virus; RT, reverse transcription; PF, forward primer; PR, reverse primer.

Main Article

Page created: November 19, 2014
Page updated: November 19, 2014
Page reviewed: November 19, 2014
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