Volume 20, Number 12—December 2014
Dispatch
Evaluation of Commercially Available Serologic Diagnostic Tests for Chikungunya Virus
, Olivier Flusin, Amanda J. Panella, Bernard Tenebray, Robert Lanciotti, and Isabelle Leparc-Goffart
Table 1
Results of serologic diagnostic testing of 23 serum samples (panel A) in a study evaluating the accuracy of commercially available CHIKV test kits*
| IgM | IgG | IgM | IgG | IgM | IgG | IgM | IgG | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CHIKV | ||||||||||||||
| IgG | ||||||||||||||
| 1 | − | + | − | + | 5,120 | + | − | − | + | − | − | |||
| 2 | − | + | − | + | 1,280 | − | − | − | − | − | + | |||
| 3 | − | + | − | + | >320 | + | + | − | + | + | + | |||
| IgM + IgG | ||||||||||||||
| 4 | + | + | + | + | 320 | − | − | + | + | + | + | |||
| 5 | + | + | + | + | 40 | − | − | + | + | + | − | |||
| 6 | + | + | + | + | 2,560 | + | − | + | + | + | + | |||
| 7 | + | + | + | + | 1,280 | − | + | + | + | NA | NA | |||
| 8 | + | + | + | + | 640 | + | + | + | + | + | + | |||
| 9 | + | + | + | + | 320 | + | − | + | − | + | + | |||
| 10 | + | + | + | + | 80 | − | − | + | − | + | + | |||
| IgM | ||||||||||||||
| 11 | + | − | + | − | <10 | − | − | + | − | + | − | |||
| 12 | + | − | + | − | <10 | − | − | + | − | + | − | |||
| 13 |
+ |
− |
+ |
− |
80 |
− |
− |
+ |
− |
+ |
− |
|||
| DENV | ||||||||||||||
| IgM + IgG | ||||||||||||||
| 14 | − | − | − | − | <10 | − | − | − | − | − | − | |||
| 15 |
− |
− |
− |
− |
<10 |
− |
− |
− |
− |
− |
− |
|||
| Negative samples‡‡ | ||||||||||||||
| 16 | − | − | − | − | <10 | − | − | − | − | − | − | |||
| 17 | − | − | − | − | <10 | + | − | − | − | − | − | |||
| 18 | − | − | − | − | <10 | − | − | - | − | − | − | |||
| 19 | − | − | − | − | <10 | + | − | + | − | − | − | |||
| 20 | − | − | − | − | <10 | − | − | + | − | + | NA | |||
| 21 | − | − | − | − | <10 | − | − | − | − | − | − | |||
| 22 | − | − | − | − | <10 | − | − | − | − | − | − | |||
| 23 | − | − | − | − | <10 | − | − | − | − | − | − |
*The serum samples were obtained from IRBA (Marseille, France). CDC, Centers for Disease Control and Prevention; CHIKV, chikungunya virus; DENV, dengue virus; IRBA, French Armed Forces Biomedical Research Institute; NA, not applicable; RDT, immunochromatography test for rapid detection.
†National Reference Center for Arboviruses at IRBA.
‡National Reference Center for Arboviruses at CDC (Fort Collins, CO, USA).
§Data are CHIKV neutralization titers of serum.
¶From Standard Diagnostics Inc., Yongin-si, South Korea.
#From CTK Biotech Inc., San Diego, CA, USA.
**From IBL International, Hamburg, Germany.
††From Euroimmun, Lübeck, Germany.
‡‡Depending on the patient’s recent travel history, these samples were tested for various other viruses. Test results were negative for following arboviruses: DENV, CHIKV, West Nile virus, Toscana virus, Japanese encephalitis virus, Rift Valley fever virus, St. Louis encephalitis virus, Mayaro virus.