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Volume 20, Number 3—March 2014
Dispatch

Role of Waddlia chondrophila Placental Infection in Miscarriage

David Baud1, Geneviève Goy1, Maria-Chiara Osterheld, Antony Croxatto, Nicole Borel, Yvan Vial, Andreas Pospischil, and Gilbert GreubComments to Author 
Author affiliations: University of Lausanne, Lausanne, Switzerland (D. Baud, G. Goy, A. Croxatto, G. Greub); University Hospital, Lausanne (D. Baud, Y. Vial, G. Greub, M.-C. Osterheld); University of Zürich, Zürich, Switzerland (N. Borel, A. Pospischil)

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Figure 1

Histopathologic analysis of placentas from women tested for infection with Waddlia chondrophila. A) Patient 140, chronic endometritis with various inflammatory cells in the deciduas, including plasmocytes (arrows) (original magnification x600). B, Patient 183, polymorphonuclear cells (PMN) in a an endometrial gland (original magnification x400). C) Patient 305, chorioamnionitis with PMN extending from the chorion to the amnios (original magnification x200). D) Patient 535, PMN in the subchorial

Figure 1. . Histopathologic analysis of placentas from women tested for infection with Waddlia chondrophila. A) Patient 140, chronic endometritis with various inflammatory cells in the deciduas, including plasmocytes (arrows) (original magnification x600). B, Patient 183, polymorphonuclear cells (PMN) in a an endometrial gland (original magnification x400). C) Patient 305, chorioamnionitis with PMN extending from the chorion to the amnios (original magnification x200). D) Patient 535, PMN in the subchorial fibrin near the gestational sac (original magnification x400). Hematoxylin and eosin stain. Immunohistochemical analysis showing W. chondrophila in placental tissue. A rabbit polyclonal antibody directed against W. chondrophila was used at a dilution of 1:12,000. Detection was performed by using the ChemMate Kit (Dako, Glostrup, Denmark). Negative controls contained antibody diluent instead of primary antibody. Negative and positive control pellets were included as described (10). All highly positive cells were found in epithelium of endometrial glands. E) Patient 535 (miscarriage) (original magnification x400). F) Patient 535 (original magnification x600) G) Patient 523 (miscarriage) (original magnification x600). H) Patient 250 (control) (original magnification x600). 3-amino-9-ethylcarbazole/peroxidase stain and hematoxylin counterstain.

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1These authors contributed equally to this article.

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