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Volume 20, Number 3—March 2014


Hendra Virus Vaccine, a One Health Approach to Protecting Horse, Human, and Environmental Health

Deborah Middleton1Comments to Author , Jackie Pallister1, Reuben Klein, Yan-Ru Feng, Jessica Haining, Rachel Arkinstall, Leah Frazer, Jin-An Huang, Nigel Edwards, Mark Wareing, Martin Elhay, Zia Hashmi, John Bingham, Manabu Yamada, Dayna Johnson, John White, Adam Foord, Hans G. Heine, Glenn A. Marsh, Christopher C. Broder, and Lin-Fa Wang
Author affiliations: CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia (D. Middleton, J. Pallister, R. Klein, J. Haining, R. Arkinstall, L. Frazer, J. Bingham, D. Johnson, J. White, A. Foord, H.G. Heine, G.A. Marsh, L.-F. Wang); Uniformed Services University, Bethesda, Maryland, USA (Y.-R. Feng, C.C. Broder); Zoetis Research & Manufacturing Pty Ltd, Parkville, Victoria, Australia (J.-A. Huang, N. Edwards, M. Wareing, M. Elhay, Z. Hashmi); National Institute of Animal Health, Ibaraki, Japan (M. Yamada); Duke–NUS (Duke and the National University of Singapore) Graduate Medical School, Singapore (L.-F. Wang)

Main Article

Table 3

Summary of sample analysis data from 10 horses in the 4 efficacy trials*

Trial no, horse ID Prechallenge antibody titer Euthanized, dpc Genome detection, sample, no. dpc
Viral infectivity control
PM tissue Oral swab Rectal swab Nasal swab Urine Feces Blood Specimen No. died/no. total
1 Horse 1/1
V1 512, 1,024 8
512, 1,024

2 Guinea pig 1/4
V3 2,048, 4,096 7
V4 128, 256 8
>4,096, >4,096

3 Ferret 2/2
V6 >4,096, >4,096 7
>4,096, >4,096

4 Ferret 2/2
V8 32, 32 7
V9 16, 32 8 2–4, 7
16, 32

*A 100-μg dose of Hendra virus soluble G glycoprotein was used in trials 1, 3, and 4; a 50-μg dose was used in trial 2. Genome was detected by PCR. ID, identification; dpc, days after challenge; PM, postmortem. − indicates a negative result.

Main Article

1These authors contributed equally to this article.