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Volume 20, Number 3—March 2014
Research

Monitoring Water Sources for Environmental Reservoirs of Toxigenic Vibrio cholerae O1, Haiti

Meer T. Alam, Thomas A. Weppelmann, Chad D. Weber, Judith A. Johnson, Mohammad H. Rashid, Catherine S. Birch, Babette A. Brumback, Valery E. Madsen Beau de Rochars, J. Glenn, and Afsar AliComments to Author 
Author affiliations: University of Florida College of Public Health and Health Professions, Gainesville, Florida, USA (M.T. Alam, T.A. Weppelmann, V.E. Madsen Beau de Rochars, A. Ali); University of Florida Emerging Pathogens Institute, Gainesville (M.T. Alam, T.A. Weppelmann, C.D. Weber, J.A. Johnson, M.H. Rashid, C.S. Birch, B.A. Brumback, V.E. Madsen Beau de Rochars, J.G. Morris, Jr., A. Ali); University of Florida College of Medicine, Gainesville (M.H. Rashid, V.E. Madsen Beau de Rochars, A. Ali)

Main Article

Table 2

PCR analysis of genes of ctx –positive toxigenic Vibrio cholerae O1 strains and ctx-negative Vcholerae O1 strain

Strain PCR
 Mismatch amplification mutation assay PCR
ompW toxR tcpACL tcpAET ctxA ctxB rstRET rstRCL rstCET rstCCL ctxBCL ctxBET
Env-9* + + + - - - - - - - - -
Env-90 + + - + + + + - - - + -
Env-94 + + - + + + + - - - + -
Env-122*† + + + - - - - - - - - -
Env-383 + + - + + + + - - - + -
Env-390* + + + - - - - - - - - -
Env-114*† + + + - - - - - - - - -

*Indicates ctx-negative V. cholerae O1 isolates.
†Env-122 and env-114 were isolated from water and a shrimp sample, respectively, from a single sampling site and at a single isolation round.

Main Article

Page created: February 19, 2014
Page updated: February 19, 2014
Page reviewed: February 19, 2014
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