Volume 20, Number 3—March 2014
Research
Monitoring Water Sources for Environmental Reservoirs of Toxigenic Vibrio cholerae O1, Haiti
Table 2
PCR analysis of genes of ctx –positive toxigenic Vibrio cholerae O1 strains and ctx-negative Vcholerae O1 strain
Strain | PCR |
Mismatch amplification mutation assay PCR |
|||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ompW | toxR | tcpACL | tcpAET | ctxA | ctxB | rstRET | rstRCL | rstCET | rstCCL | ctxBCL | ctxBET | ||
Env-9* | + | + | + | - | - | - | - | - | - | - | - | - | |
Env-90 | + | + | - | + | + | + | + | - | - | - | + | - | |
Env-94 | + | + | - | + | + | + | + | - | - | - | + | - | |
Env-122*† | + | + | + | - | - | - | - | - | - | - | - | - | |
Env-383 | + | + | - | + | + | + | + | - | - | - | + | - | |
Env-390* | + | + | + | - | - | - | - | - | - | - | - | - | |
Env-114*† | + | + | + | - | - | - | - | - | - | - | - | - |
*Indicates ctx-negative V. cholerae O1 isolates.
†Env-122 and env-114 were isolated from water and a shrimp sample, respectively, from a single sampling site and at a single isolation round.