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Volume 20, Number 8—August 2014
Dispatch

Infection with Possible Precursor of Avian Influenza A(H7N9) Virus in a Child, China, 2013

Lili Ren1, Xuelian Yu1, Baihui Zhao1, Fan Wu, Qi Jin2, Xi Zhang2, and Jianwei Wang2Comments to Author 
Author affiliations: Ministry of Health Key Laboratory for Systems Biology of Pathogens, Beijing, China (L. Ren, Q. Jin, J. Wang); Institute of Pathogen Biology, Beijing (L. Ren, Q. Jin, J. Wang); Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China (X. Yu, B. Zhao, F. Wu, X. Zhang); Shanghai Jiao Tong University, Shanghai (B. Zhao)

Main Article

Table 2

Analysis on critical mammalian-adapting amino acid substitutions in H7N9 virus strains.*†

Gene
 Site
 SH/JS01
 SH/1
 SH/2
 AH/1
 Known mutations
Relationship to mammalian adaption
HA 138 A S A A S138A Mammalian host adaption
186 G G V V G186V Unknown
226 Q Q L L Q226L Unknown
228 G G G G G228S Unknown
NA 292 R K R R R292K Osteltamivir and zanamivir resistance
69–73 deletion No Yes Yes Yes Not applicable Deletion of 69–73 Increased virulence in mice
PB2 63 I I I I I63T Co-mediate with PB1 677M, to reduce pathogenicity of H5N1 viruses
89 V V V V L89V Enhanced polymerase activity and increased virulence in mice
471 T T T T T471M Viral replication, virulence, and pathogenicity
591 Q Q Q Q Q591K Adapt in mammals that compensates for the lack of PB2–627K
627 E K K K E627K Enhanced polymerase activity and increased virulence in mice
701 D D D D D701N Enhanced transmission in guinea pigs
PB1 99 H H H H H99Y Results in transmissible of H5 virus among ferrets
353 K K K K K353R Determine viral replication, virulence, and pathogenicity
368 V I V V I368V Results in transmissible of H5 virus among ferrets
566 T T T T T566A Determine viral replication, virulence, and pathogenicity
677 T T T T T677M Co-mediate with PB2 I63T to reduce pathogenicity of H5N1 viruses
PA 100 V A A A V100A Related to human adaption
356 R R R R 356R Related to human adaption
409 S N N N S409N Enhances transmission in mammals
M1 30 D D D D N30D Increased virulence in mice
215 A A A A T215A Increased virulence in mice
M2 31 N N N N S31N Reduced susceptibility to amantadine and rimantadine
NS1 42 S S S S P42S Increased virulence in mice

*HA, hemagglutinin; NA, neuraminidase; SH/JS01, A/Shanghai/JS01/2013(H7N9); SH/1, A/Shanghai/1/2013 (H7N9); SH/2, A/Shanghai/2/2013 (H7N9); AH/1, A/Anhui/1/2013 (H7N9); PB, RNA polymerase basic subunit; PA, RNA polymerase acidic subunit; M, matrix gene; NS, nonstructural gene.
†Boldface text indicates the mutant amino acids sites related to mammalian host adaption and increased virulence.

Main Article

1These authors contributed equally to this work.

2These authors contributed equally to this work and share senior authorship.

Page created: July 18, 2014
Page updated: July 18, 2014
Page reviewed: July 18, 2014
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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