Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 20, Number 8—August 2014

Infection with Possible Precursor of Avian Influenza A(H7N9) Virus in a Child, China, 2013

Lili Ren1, Xuelian Yu1, Baihui Zhao1, Fan Wu, Qi Jin2, Xi Zhang2, and Jianwei Wang2Comments to Author 
Author affiliations: Ministry of Health Key Laboratory for Systems Biology of Pathogens, Beijing, China (L. Ren, Q. Jin, J. Wang); Institute of Pathogen Biology, Beijing (L. Ren, Q. Jin, J. Wang); Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China (X. Yu, B. Zhao, F. Wu, X. Zhang); Shanghai Jiao Tong University, Shanghai (B. Zhao)

Cite This Article


During the early stage of the avian influenza A(H7N9) epidemic in China in March 2013, a strain of the virus was identified in a 4-year-old boy with mild influenza symptoms. Phylogenetic analysis indicated that this strain, which has similarity to avian subtype H9N2 viruses, may represent a precursor of more-evolved H7N9 subtypes co-circulating among humans.

Influenza A(H7N9) virus infected >400 persons in China during March 2013–April 2014 (13) in China. Although this virus does not appear to be readily transmitted from person to person, its identification in a wide geographic area of China and discovery of amino acid changes associated with mammalian adaption of the virus have caused increased concerns for a pandemic (1,2).

The origin and evolution of the H7N9 subtype have been discussed intensively based on the results of phylogenetic analysis of the available sequences (1,48). The hemagglutinin (HA) and neuraminidase (NA) genes of the H7N9 subtype that circulated among humans during 2013 were possibly introduced from wild birds that carried differing subtype H9N2 strains and then reassorted in domestic birds such as chickens (46). A/brambling/Beijing/16/2012(H9N2) (BJ16)–like virus and/or other related avian virus H9N2 strains are proposed to be the sources of the internal genes of the 2013 H7N9 subtypes (5,7). However, the precise source and evolution route of strains in human H7N9 subtypes have not been well established (46). Intermediate or precursor strains are extrapolated to exist at the interface between avian and human H7N9 subtypes (5,6), but such strains have not been identified.

Here we report the identification of a distinct strain, A/Shanghai/JS01/2013(H7N9) (SH/JS01), which was detected in a patient with mild influenza symptoms in Shanghai during March 2013, during the very early stage of the influenza A(H7N9) epidemic. Phylogenetic analysis indicates that this strain may represent an earlier precursor of the more evolved H7N9 subtypes co-circulating at low levels at the time of isolation in March 2013 thus providing insight into the evolution of H7N9 subtypes.

The Study

A mild case of influenza A(H7N9) virus infection was identified in a 4-year-old boy in a rural area of Jinshan District, Shanghai, reported on March 31. The patient had been exposed to poultry. His signs and symptoms included acute fever (maximum 39° axillary), cough, nasal drainage, and tonsillitis. A diagnosis of upper respiratory tract infection was made, and the child recovered after 5 days of antiviral drug therapy (9,10). Nasal swab specimens were positive for influenza A(H7N9) virus by using real-time RT-PCR (11), as recommended by the World Health Organization. Although his family members, unrelated workers, and chickens he may have had contact with were tested, none tested positive for influenza virus.

The whole genome sequence of the SH/JS01strain was amplified from the nasal swab specimen by using RT-PCR (primer sequences available upon request). Strict controls were used during PCR amplification; results were confirmed by another laboratory to exclude contamination with laboratory strains. We constructed maximum likelihood trees of each gene segment sequence using the general time-reversible model implemented in MEGA 5.1 (12), and estimated divergence time using the Bayesian Markov chain Monte Carlo method implemented in BEAST (v1.6.1) (13). We compared all known strains of the 2013 H7N9 subtype and other referenced influenza virus sequences deposited in GISAID ( and GenBank (Table 1 and Technical Appendix Figures 1–8).


Thumbnail of Amino acid sequence alignment of the neuraminidase (NA) stalk region. The dark circle indicates the sequence characterized in this study. The abbreviations of the sequence names are as follows: ns, northern shoveler; wf, wild waterfowl; Ck, Chicken; Sb, shorebird; mal, mallard; wb, wild bird; HK, Hong Kong; JX, Jiangxi; ZJ, Zhejiang; SH, Shanghai.

Figure. Amino acid sequence alignment of the neuraminidase (NA) stalk regionThe dark circle indicates the sequence characterized in this studyThe abbreviations of the sequence names are as follows: ns, northern shoveler; wf,...

The critical mutations in the SH/JS01 strain associated with virulence and mammalian adaption were compared to 3 prevalent H7N9 subtype reference strains: A/Shanghai/1/2013 (SH/1), A/Shanghai/2/2013 (SH/2), and A/Anhui/1/2013 (AH/1). In the HA gene of SH/JS01, the only mammalian adapting substitution observed was 138A (H3 numbering); amino acid residues involved in receptor-binding specificity showed avian-like signatures, including 186G and 226Q, which were similar to SH/1 but distinctive from SH/2 and AH/1. In the internal genes of SH/JS01, we observed some human-like and mammalian-adapting signatures, including 89V in polymerase basic subunit (PB)2, 368V in PB1, 356R in the RNA polymerase acidic subunit, 42S in nonstructural gene 1, and 30D and 215A in matrix gene 1; however, some hallmark changes involved in mammalian adaptation still showed avian signatures, including 627E and 701D in PB2 and 100V and 409S in the RNA polymerase acidic subunit (Table 2). Most strikingly, SH/JS01 retained aa 69–73 (N9 numbering) in the stalk region of the NA gene. In contrast, deletion of aa 69–73, which is considered to occur when viruses adapt to terrestrial birds, prevailed in all the known 2013 H7N9 subtype isolates (14) (Table 2, and Figure). These findings indicate that SH/JS01 is genetically distinct from all the known human influenza A(H7N9) strains and carries more avian influenza-like signatures.

Phylogenetic analysis and divergence time estimation showed that the SH/JS01 HA gene diverged in October 2005 and was closely related to SH/1; nucleotide similarity was 99.7% (online Technical Appendix Figure 1). However, the NA gene, which is most closely related to A/northern shoveler/Hong Kong/MPL133/2010(H2N9) and A/duck/Jiangxi/21714/2011(H11N9) with nucleotide similarity of 99% and 99.3%, respectively, are estimated to have diverged in September 2010, earlier than that of known strains of the 2013 H7N9 subtype (estimated to have occurred in January 2011) (Table 1; Technical Appendix Figure 2).

On the basis of internal genes, 2013 H7N9 viruses have been divided into minor (m) and major (M) clades in the phylogenetic trees, and first 9, then 27 genotypes (5,6,8). Our data showed that SH/JS01 belongs to the m-PB2|PA|M or G3 genotype (Table 1; Technical Appendix Figures 3–8); its NS gene and that of A/Chicken/Dawang/1/2011(H9N2) (DW1), shared the highest nt identity (99.4%). The SH/JS01 NS gene had an estimated divergence time as early as October 1996 (Table 1; Technical Appendix Figure 3). The closest relatives of the SH/JS01 M gene were poultry H9N2 strains A/chicken/Jiangsu/CZ1/2012 and A/chicken/Jiangsu/NTTZ/2013 (nt identity 99.7%); BJ16 (98.6%) was not closely related. The divergence time of SH/JS01 M gene is estimated as May 2011, which is earlier than most 2013 H7N9 isolates (Table 1; Technical Appendix Figure 4). The PB2 and PA genes of SH/JS01 were positioned in minor clades and showed higher identities to A/chicken/Jiangsu/MYJMF/2012(H9N2) (99.2% and 99.3%, respectively) than to BJ16 (97.1% and 98%) (Table 1; Technical Appendix Figures 5, 6). In contrast, the PB1 and NP genes of SH/JS01 belong to the major lineage (Technical Appendix Figures 7, 8). Collectively, these data indicate that, except for PB1 and NP, the internal genes of SH/JS01 are more closely related to those in poultry H9N2 viruses identified before 2013 than to BJ16, which was an H9N2 virus considered to be the donor of most of internal genes of the 2013 H7N9 virus.


SH/JS01, a distinct H7N9 virus strain identified in 2013 during the early stage of the influenza A(H7N9) epidemic in China, provided information to define the evolution of the H7N9 subtype. Although identified in an infected human, SH/JS01 has more avian-prone properties and fewer mammalian-adapting mutations than other known human 2013 H7N9 subtypes. SH/JS01 has a waterfowl-like NA gene characterized by the absence of a deletion in the NA stalk and most of its internal genes are more closely related to avian H9N2 subtype strains isolated during the 2011–2012 influenza season than to other recently emerged strains of the H7N9 subtype. Molecular clock analysis further predicted an earlier divergence time in most genes of SH/JS01. These findings indicate that SH/JS01 might be a precursor strain of the H7N9 virus that co-circulated with more evolved viruses, although we cannot exclude that SH/JS01 may have been generated independently from the other H7N9 strains by reassortment of waterfowl strains with avian H9N2 strains and then transmitted directly to a human.

The sequences of SH/JS01 contained more avian-like signatures than those of other H7N9 isolates from humans; this underscores the potential of these viruses to infect humans. The phenotypic characteristics of SH/JS01, which might describe its zoonotic potential, remain to be investigated.

It is unclear whether other SH/JS01–like viruses are still circulating in poultry in China and if so, what the potential is for their evolution and ability to infect humans. Intensive influenza surveillance and additional influenza A virus genome sequences isolated from poultry and from humans with severe and mild manifestations of infection are needed to clarify the population dynamics of H7N9 viruses.

Dr. Ren is an associate professor at the Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Beijing. Her research is focused on the etiology and pathogenesis of respiratory viruses.



We thank Adolfo García-Sastre for his helpful discussions and critical-reading of this manuscript.

This study was supported in part by grants from Chinese Ministry of Science and Technology (KJYJ-2013-01-01-01), National Major Science and Technology Project for Control and Prevention of Major Infectious Diseases of China (2012ZX10004-206), Shanghai Health and Family Planning Commission (12GWZX081, 2013QLG007, 2013QLG001, 2013QLG003), National Funds for Distinguished Young Scientists (81225014), Program for Changjiang Scholars and Innovative Research Team in University (IRT13007), and Foundation Merieux.



  1. Gao  R, Cao  B, Hu  Y, Feng  Z, Wang  D, Hu  W, Human infection with a novel avian-origin influenza A (H7N9) virus. N Engl J Med. 2013;368:188897. DOIPubMedGoogle Scholar
  2. Uyeki  TM, Cox  NJ. Global concerns regarding novel influenza A (H7N9) virus infections. N Engl J Med. 2013;368:18624. DOIPubMedGoogle Scholar
  3. World Health Organization. Avian influenza A(H7N9) virus [cited 2014 Apr 8].
  4. Liu  D, Shi  W, Shi  Y, Wang  D, Xiao  H, Li  W, Origin and diversity of novel avian influenza A H7N9 viruses causing human infection: phylogenetic, structural, and coalescent analyses. Lancet. 2013;381:192632. DOIPubMedGoogle Scholar
  5. Wu  A, Su  C, Wang  D, Peng  Y, Liu  M, Hua  S, Sequential reassortments underlie diverse influenza H7N9 genotypes in China. Cell Host Microbe. 2013;14:44652. DOIPubMedGoogle Scholar
  6. Lam  TT, Wang  J, Shen  Y, Zhou  B, Duan  L, Cheung  CL, The genesis and source of the H7N9 influenza viruses causing human infections in China. Nature. 2013;502:2414. DOIPubMedGoogle Scholar
  7. Feng  Y, Mao  H, Xu  C, Jiang  J, Chen  Y, Yan  J, Origin and characteristics of internal genes affect infectivity of the novel avian-origin influenza A(H7N9) virus. PLoS ONE. 2013;8:e81136. DOIPubMedGoogle Scholar
  8. Cui  L, Liu  D, Shi  W, Pan  J, Qi  X, Li  X, Dynamic reassortments and genetic heterogeneity of the human-infecting influenza A (H7N9) virus. Nat Commun. 2014;5:3142–50.
  9. Ip  DK, Liao  Q, Wu  P, Gao  Z, Cao  B, Feng  L, Detection of mild to moderate influenza A/H7N9 infection by China's national sentinel surveillance system for influenza-like illness: case series. BMJ. 2013;346:f3693. DOIPubMedGoogle Scholar
  10. Yu  X, Zhang  X, He  Y, Wu  HY, Gao  X, Pan  QC, Mild infection of a novel H7N9 avian influenza virus in children in Shanghai. Emerg Microbes Infect. 2013;2:e41.
  11. World Health Organization. Real-time RT-PCR protocol for the detection of avian influenza A(H7N9) virus [cited 2013 Apr 15]. cnic_realtime_rt_pcr_protocol_a_h7n9.pdf.
  12. Tamura  K, Peterson  D, Peterson  N, Stecher  G, Nei  M, Kumar  S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:27319. DOIPubMedGoogle Scholar
  13. Drummond  AJ, Rambaut  A. BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol. 2007;7:214. DOIPubMedGoogle Scholar
  14. Cheung  CL, Vijaykrishna  D, Smith  GJ, Fan  XH, Zhang  JX, Bahl  J, Establishment of influenza A virus (H6N1) in minor poultry species in southern China. J Virol. 2007;81:1040212. DOIPubMedGoogle Scholar




Cite This Article

DOI: 10.3201/eid2008.140325

1These authors contributed equally to this work.

2These authors contributed equally to this work and share senior authorship.

Table of Contents – Volume 20, Number 8—August 2014

EID Search Options
presentation_01 Advanced Article Search – Search articles by author and/or keyword.
presentation_01 Articles by Country Search – Search articles by the topic country.
presentation_01 Article Type Search – Search articles by article type and issue.



Please use the form below to submit correspondence to the authors or contact them at the following address:

Jianwei Wang, MOH Key Laboratory for Systems Biology of Pathogens and Christophe Mérieux Laboratory, Institute of Pathogen Biology, Chinese Academy of Medical Sciences, #9 Dong Dan San Tiao, Dongcheng District, Beijing, 100730, P.R. China

Send To

10000 character(s) remaining.


Page created: July 18, 2014
Page updated: July 18, 2014
Page reviewed: July 18, 2014
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.