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Volume 21, Number 1—January 2015
Dispatch

WU Polyomavirus in Respiratory Epithelial Cells from Lung Transplant Patient with Job Syndrome

Erica A. Siebrasse, Diana V. Pastrana, Nang L. Nguyen, Annie Wang, Mark J. Roth, Steven M. Holland, Alexandra F. Freeman, John McDyer, Christopher B. Buck, and David WangComments to Author 
Author affiliations: Washington University School of Medicine, St. Louis, Missouri, USA (E.A. Siebrasse, N.L. Nguyen, A. Wang, D. Wang); National Institutes of Health, Bethesda, Maryland, USA (D.V. Pastrana, M.J. Roth, S.M. Holland, A.F. Freeman, C.B. Buck); University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA (J. McDyer)

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Figure 2

WU polyomavirus antigen in respiratory epithelial cells from lungs transplanted into a recipient (28-year-old woman) with Job syndrome. Immunofluorescence of 293T cells transfected with pDEST26-WU–virus protein 1 and stained with A) WU virus protein 1 polyclonal antibody (NN-Ab01) or B) preimmune serum. C) Double immunofluorescence with NN-Ab01 (red) and a monoclonal antibody against cytokeratin (green) showing a double-positive cell from the bronchoalveolar lavage specimen. D) Bronchoalveolar l

Figure 2. WU polyomavirus antigen in respiratory epithelial cells from lungs transplanted into a recipient (28-year-old woman) with Job syndrome. Immunofluorescence of 293T cells transfected with pDEST26-WU–virus protein 1 and stained with A) WU virus protein 1 polyclonal antibody (NN-Ab01) or B) preimmune serum. C) Double immunofluorescence with NN-Ab01 (red) and a monoclonal antibody against cytokeratin (green) showing a double-positive cell from the bronchoalveolar lavage specimen. D) Bronchoalveolar lavage specimen with multiple WU virus protein 1/cytokeratin double-positive cells. Original magnifications ×100 in panels A and B, ×600 in panel C, and ×400 in panel D.

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Page created: December 19, 2014
Page updated: December 19, 2014
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