Volume 21, Number 12—December 2015
High Prevalence of Intermediate Leptospira spp. DNA in Febrile Humans from Urban and Rural Ecuador
|Location, year||No. samples analyzed||Leptospira spp.–positive samples
||No. (%) spurious PCR products†|
|No. (%)||Species||No. (%)||Species|
|Esmeraldas, 2011–2012‡||108§||3 (2.7)||L. noguchii||73 (68)||L. wolffii||6 (4)|
|Portoviejo, 2012#||100||0||–||24 (24)||L. wolffii||0|
|Guayaquil 2011**||154||3 (1.9)||L. borgpetersenii||28 (18)||L. wolffii||9 (21)|
|1 (0.6)||L. kirschneri/L. interrogans††||–||–||0|
*The 3 communities were in rural (Esmeraldas), semiurban (Portoviejo), and urban (Guayaquil) locations. Leptospiral DNA in patient samples was detected by PCR. Molecular methods were used to amplify and sequence the leptospiral rrs gene from DNA. –, not applicable/no value.
†The spurious products represent serum samples that produced amplicons of the correct size but with DNA sequences different from Leptospira (for the pathogenic and intermediate cluster).
‡Of samples from Esmeraldas, 27% were positive for dengue virus (IgM ELISA) and Leptospira sp. (PCR).
¶Blood spot samples.
#Sixty-six samples were tested for dengue virus by IgM ELISA (57 negative, 9 positive) but were not tested for Leptospira sp.; 34 samples were IgM ELISA–positive for Leptospira sp. but were not tested for dengue virus.
**Samples tested negative for dengue virus by IgM ELISA.
††The amplicon showed the same degree of identity to both species.