Volume 21, Number 12—December 2015
Research
High Prevalence of Intermediate Leptospira spp. DNA in Febrile Humans from Urban and Rural Ecuador
Jorge Chiriboga, Verónica Barragan, Gabriela Arroyo, Andrea Sosa, Dawn N. Birdsell, Karool España, Ana Mora, Emilia Espín, María Eugenia Mejía, Melba Morales, Carmina Pinargote, Manuel Gonzalez, Rudy Hartskeerl, Paul Keim, Gustavo Bretas, Joseph N.S. Eisenberg, and Gabriel Trueba
Table 3
Species and cluster of leptospiral DNA sequences recovered from animals in 2009 and 2013, Portoviejo, Ecuador*
| Location, year, animal, no analyzed samples | Leptospira spp.–positive samples |
No. (%) spurious PCR products† | ||||
|---|---|---|---|---|---|---|
| Pathogenic cluster |
Intermediate cluster |
|||||
| No. (%) | Species | No. (%) | Species | |||
| Portoviejo, 2009‡ | ||||||
| Cattle, n = 7 | 3 (43) | L. borgpetersenii | 4 (57) | L. inadai | 0 | |
| Rats, n = 6 | 1 (17) | L. borgpetersenii | 5 (83) | L. inadai | 0 | |
| Dogs, n = 3 | 0 | – | 3 (100) | L. inadai | 0 | |
| Pigs, n = 3 |
1 (33) |
L. borgpetersenii |
2 (67) |
L. inadai |
0 |
|
| Portoviejo, 2013§ | ||||||
| Cattle, n = 26 | 5 (19) | L. borgpetersenii | 1 (4) | L.wolffii | 3 (27) | |
| 1 (4) | L. kirschneri | – | – | |||
| Rats, n = 60 | 3 (5) | L. borgpeterseni | 1 (1.7) | L.wolffii | 3 (21) | |
| 2 (3.3) | L. kirschner¶ | – | – | |||
| Pigs, n = 30 |
2 (6.7) |
L. borgpetersenii |
1 (3.3) |
L.wolffii |
5 (50) |
|
| *–, not applicable/no value. †Percentage of amplicons (obtained from samples of each animal species) which showed expected size but the DNA sequences were different from Leptospira spp. ‡Dry season. §Rainy season. ¶One amplicon also showed the same degree of identity to L. interrogans. | ||||||
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Page updated: November 16, 2015
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