Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 21, Number 12—December 2015

High Prevalence of Intermediate Leptospira spp. DNA in Febrile Humans from Urban and Rural Ecuador

Jorge Chiriboga, Verónica Barragan, Gabriela Arroyo, Andrea Sosa, Dawn N. Birdsell, Karool España, Ana Mora, Emilia Espín, María Eugenia Mejía, Melba Morales, Carmina Pinargote, Manuel Gonzalez, Rudy Hartskeerl, Paul Keim, Gustavo Bretas, Joseph N.S. Eisenberg, and Gabriel TruebaComments to Author 
Author affiliations: Microbiology Institute, Universidad San Francisco de Quito, Campus Cumbaya, Quito, Ecuador (J. Chiriboga, V. Barragan, G. Arroyo, A. Sosa, E. Espín, M.E. Mejía, G. Trueba); Northern Arizona University, Flagstaff, Arizona, USA (D.N. Birdsell, P. Keim); Instituto Nacional de Salud Pública e Investigación, Portoviejo, Ecuador (K. España, A. Mora, M. Morales, C. Pinargote, M. Gonzalez); Ministerio de Salud Pública, Portoviejo (M. Morales); Royal Tropical Institute (KIT), Amsterdam, the Netherlands (R. Hartskeerl); Organización Panamericana de la Salud OPS, Guayaquil, Ecuador (G. Bretas); University of Michigan, Ann Arbor, Michigan, USA (J.N.S. Eisenberg)

Main Article

Table 3

Species and cluster of leptospiral DNA sequences recovered from animals in 2009 and 2013, Portoviejo, Ecuador*

Location, year, animal, no analyzed samples Leptospira spp.–positive samples
No. (%) spurious PCR products†
Pathogenic cluster
Intermediate cluster
No. (%) Species No. (%) Species
Portoviejo, 2009‡
Cattle, n = 7 3 (43) L. borgpetersenii 4 (57) L. inadai 0
Rats, n = 6 1 (17) L. borgpetersenii 5 (83) L. inadai 0
Dogs, n = 3 0 3 (100) L. inadai 0
Pigs, n = 3
1 (33)
L. borgpetersenii

2 (67)
L. inadai
Portoviejo, 2013§
Cattle, n = 26 5 (19) L. borgpetersenii 1 (4) L.wolffii 3 (27)
1 (4) L. kirschneri
Rats, n = 60 3 (5) L. borgpeterseni 1 (1.7) L.wolffii 3 (21)
2 (3.3) L. kirschner¶
Pigs, n = 30
2 (6.7)
L. borgpetersenii

1 (3.3)
5 (50)
*–, not applicable/no value.
†Percentage of amplicons (obtained from samples of each animal species) which showed expected size but the DNA sequences were different from Leptospira spp.
‡Dry season.
§Rainy season.
¶One amplicon also showed the same degree of identity to L. interrogans.

Main Article

Page created: November 16, 2015
Page updated: November 16, 2015
Page reviewed: November 16, 2015
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.