Volume 21, Number 12—December 2015
Genetic Characterization of Highly Pathogenic Avian Influenza A(H5N6) Virus, Guangdong, China
To the Editor: Since the first detection of the influenza A(H5N1) virus in geese in China during 1996 (http://www.cdc.gov/flu/avianflu/h5n1-virus.htm), H5 subtype viruses have continued to reassort and evolve, giving rise to multiple virus clades and gene constellations. Recently, clade 22.214.171.124 viruses have shown a predilection for genetic reassortment, giving rise to H5N2, H5N5, H5N6, and H5N8 virus subtypes, and have become globally widespread, causing infections in wild birds or poultry elsewhere in Asia, and in Europe and North America (1–3). The H5N6 subtype viruses have circulated in China since 2013 and have been mainly identified in ducks or chickens in the southern (Jiangxi, Guangdong) or western (Sichuan) areas (4,5). Two lineages of H5N6 viruses with distant genetic background were found among the H5N6 viruses isolated in China (5).
In China, there have been 3 cases of H5N6 virus infection among humans, causing 2 deaths. We recently reported the clinical characteristics and progression of a patient infected by the H5N6 virus in Guangzhou City, China, who was the second reported case-patient infected with this subtype (6). After having contact with poultry, he began to manifest an influenza-like illness on December 3, 2014, and progressed to a primary viral pneumonia. The H5N6 virus A/Guangzhou/39715/2014 (GenBank accession nos. KP765785–KP765792) was isolated from a throat swab specimen collected on day 8 of his illness by inoculation into 9–11-day-old, specific pathogen-free embryonated chicken eggs. He recovered from his infection and was discharged from the hospital on day 58.
Multiple sequence alignments showed that the hemagglutinin (HA) and neuraminidase (NA) genes of A/Guangzhou/39715/2014 shared the highest nucleotide identity with A/chicken/Dongguan/2690/2013 (H5N6) (99.4% and 98.3%, respectively) (Technical Appendix 1). All internal genes were also closely related to A/chicken/Dongguan/2690/2013 (H5N6), ranging from 98.5% nucleotide identity for the polymerase acidic (PA) gene and 100.0% for the matrix (M) gene. The genome segments were also 98.2%–99.7% identical to A/duck/Guangdong/GD012014 and 98.3%–99.4% identical to A/chicken/Laos/LPQ001/2014, which caused outbreaks in domestic ducks and poultry, respectively, indicating that these viruses have the same genotype.
HA gene phylogeny confirmed that this virus belonged to clade 126.96.36.199 (Technical Appendix 1, Figure 4). Notably, the HA genes of the H5N1, H5N2, and H5N8 viruses that were recently detected in wild birds in North America also belong to this clade, indicating that viruses from this clade are becoming globally widespread. More specifically, this isolate clustered within a sublineage that includes H5N6 isolates from poultry from Guangdong and Jiangxi provinces, China, and from Laos (5,7). The A/Sichuan/26221/2014 (H5N6) virus that recently caused a fatal human infection in Sichuan Province, China is also within clade 188.8.131.52, but clusters in a distinct sub-lineage (Figure, panel A).
The HA cleavage site of both human isolates contained multiple basic amino acids, suggesting that that they are highly pathogenic avian influenza viruses. Amino acid substitutions E190D, Q226L, or G228S (H3 numbering) in the HA gene that are known to enhance binding to mammalian receptors were not found. The NA gene phylogeny showed that A/Guangzhou/39715/2014 is likely originated from group II lineage influenza A(H6N6) viruses that circulate among domestic ducks in China (8) (Figure, panel B). An 11-aa deletion at the residue 59–69 position of the NA protein was identified in the isolate of this study, in the other H5N6 viruses of the same cluster, and in an H4N6 virus isolate from a duck in Shanghai, China. This deletion was monophyletic and likely originated from A/swine/Guangdong/K6/2010 (H6N6)–like viruses (Figure, panel B). However, it was not observed in other 184.108.40.206 viruses, such as A/Sichuan/26221/2014.
No mutations associated with oseltamivir or amantadine resistance was found in NA or M2 genes. The internal genes of the current H5N6 isolate were similar to 220.127.116.11b H5N1 subtype viruses found in domestic ducks from south-central and eastern China (5,7–10 [Technical Appendix 1]). The 6 internal genes are 97%–99% homologous to another isolate from a human, A/Sichuan/26221/2014, suggesting that the internal genes of the viruses may be reassorted from a common origin.
The phylogenetic clustering observed for the HA gene was also conserved for the internal genes. In contrast with all avian viruses within this clade, the current human isolate contains the mammalian adaptation mutation PB2-E627K, and A/Sichuan/26221/2014 has acquired PB2-D701N, suggesting a rapid acquisition of mammalian adaptation changes that likely arose after human infection.
There is still limited information on human disease caused by the emerging H5 lineage. Our genetic analysis suggests that the H5N6 virus isolated from the patient is originated from the avian host. Although the genetic background of H5N6 virus isolated from the third case in Yunnan Province, China, on January 2015 is still not known, the isolates from the human cases of H5N6 infection reported to date show distant genetic diversity, indicating that viruses from both clusters may pose a threat to humans. This rapidly evolving and globally spreading virus lineage thus provides a threat to global public health.
We thank the authors and originating and submitting laboratories of the sequences in the EpiFlu Database from Global Initiative on Sharing Avian Influenza Data, on which this research is based (Technical Appendix 2).
This study was supported by Municipal Science and Technology Bureau Foundation of Guangzhou (2014Y2-00031), National Science and Technology Major Project of the Ministry of Science and Technology of China (2014ZX10004006), Science research project of the Guangdong Province (2013B020224006), the Area of Excellence Scheme of the Hong Kong University Grants Committee (AoE/M-12/06) of the Government of Hong Kong Special Administrative Region, China and Contract HHSN272201400006C from the National Institute of Allergy and Infectious Disease, National Institutes of Health, USA.
- World Health Organization. Antigenic and genetic characteristics of zoonotic influenza viruses and development of candidate vaccine viruses for pandemic preparedness [cited 2015 Feb 27]. http://www.who.int/influenza/vaccines/virus/201502_zoonotic_vaccinevirusupdate.pdf?ua=1
- European Food Safety Authority. Highly pathogenic avian influenza A subtype H5N8. EFSA Journal. 2014;12:3941–72.
- Centers for Disease Control and Prevention. Outbreaks of avian influenza A (H5N2), (H5N8), and (H5N1) among birds—-United States, December 2014–January 2015. MMWR Morb Mortal Wkly Rep. 2015;64:111 .
- Shen H, Wu B, Chen Y, Bi Y, Xie Q, Influenza A. H5N6) virus reassortant, southern China, 2014. Emerg Infect Dis. 2015;21:1261–2.
- Bi Y, Mei K, Shi W, Liu D, Yu X, Gao Z, Two novel reassortants of avian influenza A(H5N6) virus in China. J Gen Virol. 2015;96:975–81 .
- Yang ZF, Mok CKP, Peiris JSM, Zhong NS. Human disease caused by novel reassortant highly pathogenic avian influenza A H5N6 virus: A case report. N Engl J Med. 2015. In press.
- Wang G, Deng G, Shi J, Luo W, Zhang G, Zhang Q, H6 influenza viruses pose a potential threat to human health. J Virol. 2014;88:3953–64.
- Wong FY, Phommachanh P, Kalpravidh W, Chanthavisouk C, Gilbert J, Bingham J, Reassortant highly pathogenic influenza A(H5N6) virus in Laos. Emerg Infect Dis. 2015;21:511–6.
- Hai-bo W, Chao-tan G, Ru-feng L, Li-hua X, En-kang W, Jin-biao Y, Characterization of a highly pathogenic H5N1 avian influenza virus isolated from ducks in eastern China in 2011. Arch Virol. 2012;157:1131–6.
- Deng G, Tan D, Shi J, Cui P, Jiang Y, Liu L, Complex reassortment of multiple subtypes of avian influenza viruses in domestic ducks at the Dongting Lake region of China. J Virol. 2013;87:9452–62.
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1These authors contributed equally to this article.