Volume 21, Number 2—February 2015
Research
Novel Reassortant Influenza A(H5N8) Viruses among Inoculated Domestic and Wild Ducks, South Korea, 2014
Table 2
Virus isolation from swab samples obtained from 2 Baikal teal ducks inoculated with influenza A/broilerduck/Kr/Buan2/2014 (H5N8) virus*
Virus titer, log10 TCID50/0.1 mL (no. positive/no. inoculated)† |
HI titer‡ | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Oropharyngeal samples, days postinoculation |
Cloacal samples, days postinoculation |
||||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 10 | 14 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 10 | 14 | ||
4.5 (1/2) | 4.2 (1/2) | 0 (0/1) | 0 (0/1) | 0 (0/1) | 0 (0/1) | 0 (0/1) | 0 (0/1) | 0 (0/1) | 0 (0/2) | 1.2 (1/2) | 0 (0/1) | 0 (0/1) | 0 (0/1) | 0 (0/1) | 0 (0/1) | 0 (0/1) | 0 (0/1) | 1/1 |
*Birds were inoculated with 106.5 50% egg-infective dose/0.1 mL of virus via the intranasal route, and oropharyngeal and cloacal swab samples were collected on the indicated day. No clinical signs of illness were observed in the birds; however, 1 bird died on day 3 post-inoculation; TCID, tissue culture infective dose; HI, hemagglutination inhibition.
†Virus was detected only in the bird that died.
‡HI titer was assayed in a serum sample taken at day 14 postinoculation; data shows the ratio of the number of antibody-positive animals to the number of virus-inoculated animals.