Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 21, Number 3—March 2015
Letter

Porcine Epidemic Diarrhea Virus Replication in Duck Intestinal Cell Line

Mahesh KhatriComments to Author 
Author affiliation: Ohio State University, Wooster, Ohio, USA

Main Article

Figure

Replication of porcine epidemic diarrhea virus (PEDV) in a newly established immortalized duck intestinal epithelial cell line (MK-DIEC) infected with PEDV at a multiplicity of infection of 0.1 in the presence of different concentrations of trypsin. A) Twenty-four hours after infection, PEDV nucleoprotein in infected cells was detected by immunofluorescence assay using fluorescein isothiocyanate–labeled nucleoprotein-specific monoclonal antibody. B) PEDV-induced cytopathic effect in MK-DIEC cell

Figure. Replication of porcine epidemic diarrhea virus (PEDV) in a newly established immortalized duck intestinal epithelial cell line (MK-DIEC) infected with PEDV at a multiplicity of infection of 0.1 in the presence of different concentrations of trypsin. A) Twenty-four hours after infection, PEDV nucleoprotein in infected cells was detected by immunofluorescence assay using fluorescein isothiocyanate–labeled nucleoprotein-specific monoclonal antibody. B) PEDV-induced cytopathic effect in MK-DIEC cells 36 h after infection.

Main Article

Page created: February 18, 2015
Page updated: February 18, 2015
Page reviewed: February 18, 2015
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external