Volume 21, Number 4—April 2015
La Crosse Virus in Aedes japonicus japonicus Mosquitoes in the Appalachian Region, United States
|Collection date||County, state||Trap type†||Pool size‡||LACV detection method||Ct value||MLE (95% CI)|
|2005 Aug||Wise, VA||Ovitrap||9||RT-PCR||38.04||8.59 (0.54 –41.00)|
|2008 Jul||Montgomery, VA||Gravid||22||RT-PCR||37.57||4.51 (0.26–22.00)|
|2009 Jul||Montgomery, VA||Gravid||3||Isolation, RT-PCR||14.00||0.23 (0.01–1.11)|
|2009 Jul||Craig, VA||Gravid||50||Isolation, RT-PCR||23.00||1.28 (0.07–6.29)|
|2013 Jun||Fayette, WV||Multiple adult||36||RT-PCR||37.66||13.41 (5.18–29.14)|
|2013 Jul||Cabell, WV||Multiple adult||1||RT-PCR||34.72||13.41 (5.18–29.14)|
|2013 Aug||Fayette, WV||Multiple adult||15||RT-PCR||37.35||13.41 (5.18–29.14)|
|2013 Aug||Fayette, WV||Multiple adult||2||RT-PCR||34.64||13.41 (5.18–29.14)|
|2013 Sep||Kanawha, WV||Multiple adult||1||RT-PCR||37.43||13.41 (5.18–29.14)|
*Ct, cycle threshold; LACV, La Crosse virus; MLE, maximum-likelihood estimate of the proportion of infected mosquitoes; RT-PCR, reverse transcription PCR.
†All mosquitoes were collected from the field as adults except when ovitraps were used to collect eggs. Adults were reared from the field-collected eggs before testing for arboviruses. Multiple adult traps were gravid traps, carbon dioxide–emitting light traps, and BG Sentinel (Biogents AG, Regensburg, Germany) traps baited with octenol lures.
‡Pool size indicates no. adult mosquitoes tested for LACV.
1Current affiliation: United States Geological Survey, Reston, Virginia, USA.