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Volume 21, Number 4—April 2015

La Crosse Virus in Aedes japonicus japonicus Mosquitoes in the Appalachian Region, United States

M. Camille Harris1Comments to Author , Eric J. Dotseth, Bryan Jackson, Steven D. Zink, Paul E. Marek, Laura D. Kramer, Sally L. Paulson, and Dana M. Hawley
Author affiliations: Virginia Polytechnic Institute and State University , Blacksburg, Virginia, USA (M.C. Harris, B.T. Jackson, P.E. Marek, S.L. Paulson, D.M. Hawley); West Virginia Department of Health and Human Resources, Charleston, West Virginia, USA (E.J. Dotseth); New York State Department of Health, Slingerlands, New York, USA (S.D. Zink, L.D. Kramer)

Main Article


Detection of LACV RNA and virus isolations in Aedes japonicus mosquito pools from Virginia and West Virginia, USA*

Collection date County, state Trap type† Pool size‡ LACV detection method Ct value MLE (95% CI)
2005 Aug Wise, VA Ovitrap 9 RT-PCR 38.04 8.59 (0.54 –41.00)
2008 Jul Montgomery, VA Gravid 22 RT-PCR 37.57 4.51 (0.26–22.00)
2009 Jul Montgomery, VA Gravid 3 Isolation, RT-PCR 14.00 0.23 (0.01–1.11)
2009 Jul Craig, VA Gravid 50 Isolation, RT-PCR 23.00 1.28 (0.07–6.29)
2013 Jun Fayette, WV Multiple adult 36 RT-PCR 37.66 13.41 (5.18–29.14)
2013 Jul Cabell, WV Multiple adult 1 RT-PCR 34.72 13.41 (5.18–29.14)
2013 Aug Fayette, WV Multiple adult 15 RT-PCR 37.35 13.41 (5.18–29.14)
2013 Aug Fayette, WV Multiple adult 2 RT-PCR 34.64 13.41 (5.18–29.14)
2013 Sep Kanawha, WV Multiple adult 1 RT-PCR 37.43 13.41 (5.18–29.14)

*Ct, cycle threshold; LACV, La Crosse virus; MLE, maximum-likelihood estimate of the proportion of infected mosquitoes; RT-PCR, reverse transcription PCR.
†All mosquitoes were collected from the field as adults except when ovitraps were used to collect eggs. Adults were reared from the field-collected eggs before testing for arboviruses. Multiple adult traps were gravid traps, carbon dioxide–emitting light traps, and BG Sentinel (Biogents AG, Regensburg, Germany) traps baited with octenol lures.
‡Pool size indicates no. adult mosquitoes tested for LACV.

Main Article

1Current affiliation: United States Geological Survey, Reston, Virginia, USA.

Page created: March 17, 2015
Page updated: March 17, 2015
Page reviewed: March 17, 2015
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