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Volume 21, Number 4—April 2015
Dispatch

Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa

Murielle Baltazar12, Antoinette Ngandjio1, Kathryn Elizabeth Holt, Elodie Lepillet, Maria Pardos de la Gandara3, Jean-Marc Collard, Raymond Bercion4, Ariane Nzouankeu, Simon Le Hello, Gordon Dougan, Marie-Christine Fonkoua, and François-Xavier WeillComments to Author 
Author affiliations: Institut Pasteur, Paris, France (M. Baltazar, E. Lepillet, M. Pardos de la Gandara, S. Le Hello, F.-X. Weill); Centre Pasteur du Cameroun, Yaoundé, Cameroon (A. Ngandjio, A. Nzouankeu, M.-C. Fonkoua); University of Melbourne, Melbourne, Victoria, Australia (K.E. Holt); Wellcome Trust Sanger Institute, Cambridge, UK (K.E. Holt, G. Dougan); Scientific Institute of Public Health, Brussels, Belgium (J.-M. Collard); Institut Pasteur de Bangui, Bangui, Central African Republic (R. Bercion)

Main Article

Figure 1

Characteristics of 50 Salmonella enterica serotype Typhi isolates. The dendrogram was generated by using BioNumerics version 6.6 software (Applied Maths, Sint-Martens-Latem, Belgium) and shows results of cluster analysis on the basis of XbaI–pulsed-field gel electrophoresis (PFGE) fingerprinting. Similarity analysis was performed by using the Dice coefficient, and clustering analysis was performed by using UPGMA. CRISPR1, clustered regularly interspaced short palindromic repeats 1; MDR, multidru

Figure 1. Characteristics of 50 Salmonella enterica serotype Typhi isolates. The dendrogram was generated by using BioNumerics version 6.6 software (Applied Maths, Sint-Martens-Latem, Belgium) and shows results of cluster analysis on the basis of XbaI–pulsed-field gel electrophoresis (PFGE) fingerprinting. Similarity analysis was performed by using the Dice coefficient, and clustering analysis was performed by using UPGMA. CRISPR1, clustered regularly interspaced short palindromic repeats 1; MDR, multidrug resistant; AMX, amoxicillin; STR, streptomycin; SXT, trimethoprim/sulfamethoxazole; CHL, chloramphenicol; TET, tetracycline; SPT, spectinomycin; SSS, sulfamethoxazole. Zaire is the former name of the Democratic Republic of the Congo. Plasmid multilocus sequence typing (pMLST) was performed as described for incN http://pubmlst.org/plasmid/) or incHI1 (4). Plasmid size was estimated by using S1 nuclease PFGE (14). For isolates from Africa, red indicates linage A, blue indicates lineage B, and green indicates lineage C.

Main Article

References
  1. Buckle  GC, Walker  CL, Black  RE. Typhoid fever and paratyphoid fever: systematic review to estimate global morbidity and mortality for 2010. J Glob Health. 2012;2:010401. DOIPubMedGoogle Scholar
  2. Roumagnac  P, Weill  FX, Dolecek  C, Baker  S, Brisse  S, Chinh  NT, Evolutionary history of Salmonella Typhi. Science. 2006;314:1301–4. DOIPubMedGoogle Scholar
  3. Holt  KE, Parkhill  J, Mazzoni  CJ, Roumagnac  P, Weill  FX, Goodhead  I, High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi. Nat Genet. 2008;40:987–93. DOIPubMedGoogle Scholar
  4. Phan  MD, Kidgell  C, Nair  S, Holt  KE, Turner  AK, Hinds  J, Variation in Salmonella enterica serovar Typhi IncHI1 plasmids during the global spread of resistant typhoid fever. Antimicrob Agents Chemother. 2009;53:716–27. DOIPubMedGoogle Scholar
  5. Holt  KE, Phan  MD, Baker  S, Duy  PT, Nga  TV, Nair  S, Emergence of a globally dominant IncHI1 plasmid type associated with multiple drug resistant typhoid. PLoS Negl Trop Dis. 2011;5:e1245. DOIPubMedGoogle Scholar
  6. Kariuki  S, Revathi  G, Kiiru  J, Mengo  DM, Mwituria  J, Muyodi  J, Typhoid in Kenya is associated with a dominant multidrug-resistant Salmonella enterica serovar Typhi haplotype that is also widespread in Southeast Asia. J Clin Microbiol. 2010;48:2171–6. DOIPubMedGoogle Scholar
  7. Akinyemi  KO, Coker  AO. Trends of antibiotic resistance in Salmonella enterica serovar typhi isolated from hospitalized patients from 1997 to 2004 in Lagos, Nigeria. Indian J Med Microbiol. 2007;25:436–7. DOIPubMedGoogle Scholar
  8. Mills-Robertson  F, Addy  ME, Mensah  P, Crupper  SS. Molecular characterization of antibiotic resistance in clinical Salmonella typhi isolated in Ghana. FEMS Microbiol Lett. 2002;215:249–53. DOIPubMedGoogle Scholar
  9. Gross  U, Amuzu  SK, de Ciman  R, Kassimova  I, Gross  L, Rabsch  W, Bacteremia and antimicrobial drug resistance over time, Ghana. Emerg Infect Dis. 2011;17:1879–82. DOIPubMedGoogle Scholar
  10. Dagnra  AY, Akolly  K, Gbadoe  A, Aho  K, David  M. Emergence of multidrug resistant Salmonella strains in Lome (Togo) [in French]. Med Mal Infect. 2007;37:266–9. DOIPubMedGoogle Scholar
  11. Lunguya  O, Lejon  V, Phoba  MF, Bertrand  S, Vanhoof  R, Verhaegen  J, . Salmonella Typhi in the Democratic Republic of the Congo: fluoroquinolone decreased susceptibility on the rise. EPLoS Negl Trop Dis. 2012; 6:e1921. Doe: . Pub 2012 Nov 5.DOIGoogle Scholar
  12. Cooke  FJ, Day  M, Wain  J, Ward  LR, Threlfall  EJ. Cases of typhoid fever imported to England, Scotland and Wales (2000–2003). Trans R Soc Trop Med Hyg. 2007;101:398–404. DOIPubMedGoogle Scholar
  13. Loury  P, Tillaut  H, Faisant  M, Paillereau  N, Marquis  M, Mari  C, Cluster of typhoid fever cases in Ille-et-Vilaine (France), April 2009 [in French]. Bull Epidémiol Hebd. 2010;44:446–8.
  14. Le Hello  S, Harrois  D, Bouchrif  B, Sontag  L, Elhani  D, Guibert  V, Highly drug-resistant Salmonella enterica serotype Kentucky ST198-X1: a microbiological study. Lancet Infect Dis. 2013;13:672–9. DOIPubMedGoogle Scholar
  15. Fabre  L, Le Hello  S, Roux  C, Issenhuth-Jeanjean  S, Weill  FX. CRISPR is an optimal target for the design of specific PCR assays for Salmonella enterica serotypes Typhi and Paratyphi A. PLoS Negl Trop Dis. 2014;8:e2671 . DOIPubMedGoogle Scholar

Main Article

1These authors contributed equally to this article.

2Current affiliation: University of Limoges, Limoges, France.

3Current affiliation: Rockefeller University, New York, New York, USA.

4Current affiliation: Institute Pasteur de Dakar, Dakar, Senegal.

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Page updated: March 17, 2015
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