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Volume 21, Number 7—July 2015

Monitoring of Ebola Virus Makona Evolution through Establishment of Advanced Genomic Capability in Liberia

Jeffrey R. Kugelman1, Michael R. Wiley1, Suzanne Mate1, Jason T. Ladner1, Brett Beitzel, Lawrence Fakoli, Fahn Taweh, Karla Prieto, Joseph W. Diclaro, Timothy Minogue, Randal J. Schoepp, Kurt E. Schaecher, James Pettitt, Stacey Bateman, Joseph Fair, Jens H. Kuhn, Lisa Hensley, Daniel J. Park, Pardis C. Sabeti, Mariano Sanchez-Lockhart, Fatorma K. Bolay, Gustavo PalaciosComments to Author , on behalf of US Army Medical Research Institute of Infectious DiseasesNational Institutes of HealthIntegrated Research Facility–Frederick Ebola Response Team 2014–2015
Author affiliations: US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA (J.R. Kugelman, M.R. Wiley, S. Mate, J.T. Ladner, B. Beitzel, K. Prieto, T. Minogue, R.J. Schoepp, K.E. Schaecher, S. Bateman, M. Sanchez-Lockhart, G. Palacios); Liberian Institute for Biomedical Research, Charlesville, Liberia (L. Fakoli, F. Taweh, F.K. Bolay); Naval Medical Research Unit 3, Cairo, Egypt (J.W. Diclaro); Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick (J. Pettitt, J.H. Kuhn, L. Hensley); Foundation Merieux, Washington, DC, USA (J. Fair); Broad Institute, Cambridge, Massachusetts, USA (D.J. Park, P.C. Sabeti); Center for Systems Biology, Harvard University, Cambridge (P.C. Sabeti)

Main Article

Table 1

Characteristics of Ebola virus samples from selected patients, Liberia, September 2014–February 2015*

Sample ID Patient age, y/sex County of residence Test date Sample type Average Ct value†
LIBR10054 53/M Bomi 2014 Sep 23 Plasma 20.5
LIBR10053 42/NA Not Available 2014 Oct 1 NA 22
LIBR0058 67/M Rivercess 2014 Nov 5 NA 22
LIBR0059 27/M Rivercess 2014 Nov 5 NA 22
LIBR0073 27/M Grand Bassa 2014 Nov 6 Plasma 18.5
LIBR0067 29/NA Bomi 2014 Nov 6 Plasma 21
LIBR0063 3/F Montesserrado 2014 Nov 6 Oral swab 17.5
LIBR0093 47/M Montesserrado 2014 Nov 6 Plasma 15.5
LIBR0092 18/F Montesserrado 2014 Nov 8 Plasma 21
LIBR0090 62/F Margibi 2014 Nov 8 Plasma 22
LIBR0116 4/F Grand Bassa 2014 Nov 10 Plasma 19
LIBR0168 15/M Bomi 2014 Nov 13 Plasma 22.5
LIBR0176 42/M Montesserrado 2014 Nov 14 Oral swab 22.5
LIBR0173 64/M Montesserrado 2014 Nov 14 Oral swab 22
LIBR0286 9/F Grand Cape Mount 2014 Nov 22 Plasma 22
LIBR0333 35/F Grand Cape Mount 2014 Nov 25 Plasma 19.5
LIBR0423 45/F Montesserrado 2014 Dec 3 Plasma 21.5
LIBR0430 1/M Grand Bassa 2014 Dec 3 Oral swab 23.5
LIBR0503 8/F Sinoe 2014 Dec 10 Plasma 23
LIBR0505 29/F Sinoe 2014 Dec 10 Plasma 25
LIBR0605 2/M Montesserrado 2014 Dec 20 Oral swab 23
LIBR0624 53/M Montesserrado 2014 Dec 22 Plasma 19.5
LIBR0993 33/M Montesserrado 2015 Jan 20 Plasma 19.5
LIBR1195 35/M Margibi 2015 Feb 2 Oral swab 22.5
LIBR1413 56 M Montesserrado 2015 Feb 14 Plasma 22.5

*Ct, cycle threshold; ID, identification; NA, not available.
†Ct values used as indicator of viral load obtained from 2 diagnostic assays performed on all samples (Kulesh-TM and Kulesh-MGB [9]).

Main Article

1These authors contributed equally to this article.

Page created: June 12, 2015
Page updated: June 12, 2015
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