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Volume 21, Number 7—July 2015
Research

Monitoring of Ebola Virus Makona Evolution through Establishment of Advanced Genomic Capability in Liberia

Jeffrey R. Kugelman1, Michael R. Wiley1, Suzanne Mate1, Jason T. Ladner1, Brett Beitzel, Lawrence Fakoli, Fahn Taweh, Karla Prieto, Joseph W. Diclaro, Timothy Minogue, Randal J. Schoepp, Kurt E. Schaecher, James Pettitt, Stacey Bateman, Joseph Fair, Jens H. Kuhn, Lisa Hensley, Daniel J. Park, Pardis C. Sabeti, Mariano Sanchez-Lockhart, Fatorma K. Bolay, Gustavo PalaciosComments to Author , on behalf of US Army Medical Research Institute of Infectious DiseasesNational Institutes of HealthIntegrated Research Facility–Frederick Ebola Response Team 2014–2015
Author affiliations: US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA (J.R. Kugelman, M.R. Wiley, S. Mate, J.T. Ladner, B. Beitzel, K. Prieto, T. Minogue, R.J. Schoepp, K.E. Schaecher, S. Bateman, M. Sanchez-Lockhart, G. Palacios); Liberian Institute for Biomedical Research, Charlesville, Liberia (L. Fakoli, F. Taweh, F.K. Bolay); Naval Medical Research Unit 3, Cairo, Egypt (J.W. Diclaro); Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick (J. Pettitt, J.H. Kuhn, L. Hensley); Foundation Merieux, Washington, DC, USA (J. Fair); Broad Institute, Cambridge, Massachusetts, USA (D.J. Park, P.C. Sabeti); Center for Systems Biology, Harvard University, Cambridge (P.C. Sabeti)

Main Article

Table 2

Next-generation sequencing of 25 Ebola virus isolates derived from selected patients sampled, Liberia, September 2014–February 2015

Sample ID Coverage, %* No. reads Finishing category† GenBank accession no.
LIBR0093 99.4 169,000 Coding complete KR006947
LIBR0116 97.9 710,168 Coding complete KR006948
LIBR10054 98 2,150,725 Coding complete KR006964
LIBR0073 98.5 3,351,831 Coding complete KR006944
LIBR0503 98.9 3,193,168 Coding complete KR006956
LIBR0286 98.3 1,731,953 Coding complete KR006952
LIBR0993 96.5 750,000 Standard draft KR006960
LIBR0423 97.1 2,676,454 Standard draft KR006954
LIBR0333 97.1 1,775,653 Standard draft KR006953
LIBR10053 98 1,691,652 Standard draft KR006963
LIBR0067 97 2,403,590 Standard draft KR006943
LIBR0092 93.9 2,758,142 Standard draft KR006946
LIBR0090 93.1 1,422,271 Standard draft KR006945
LIBR1413 88.2 2,500,000 Standard draft KR006962
LIBR0058 91.4 1,632,978 Standard draft KR006940
LIBR0176 89.4 1,907,863 Standard draft KR006951
LIBR0168 89.2 1,221,075 Standard draft KR006949
LIBR0505 83.8 741,165 Standard draft KR006957
LIBR1195 73.1 2,200,773 Standard draft KR006961
LIBR0624 68 1,550,511 Standard draft KR006959
LIBR0063 69 2,883,384 Standard draft KR006942
LIBR0173 72.3 1,456,490 Standard draft KR006950
LIBR0059 59.1 851,606 Standard draft KR006941
LIBR0605 64.7 1,587,732 Standard draft KR006958
LIBR0430 56.2 3,139,009 Standard draft KR006955

*Percentage of genome bases (of 18,959 total bases) called in the consensus sequences (requires >3× coverage with base quality >20).
†Categories are defined in (19).

Main Article

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Main Article

1These authors contributed equally to this article.

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