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Volume 21, Number 8—August 2015
Dispatch

Enterovirus A71 Meningoencephalitis Outbreak, Rostov-on-Don, Russia, 2013

Ludmila V. Akhmadishina, Marina V. Govorukhina, Evgeniy V. Kovalev, Svetlana A. Nenadskaya, Olga E. Ivanova, and Alexander N. LukashevComments to Author 
Author affiliations: Chumakov Institute of Poliomyelitis and Viral Encephalitides, Moscow, Russia (L.V. Akhmadishina, O.E. Ivanova, A.N. Lukashev); Center of Hygiene and Epidemiology of Rostov Region, Rostov-on-Don, Russia (M.V. Govorukhina); Administration of Russian Federal Consumer Rights Protection and Human Health Control Service of Rostov Region, Rostov-on-Don (E.V. Kovalev, S.A. Nenadskaya)

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Figure 2

Phylogenetic tree comparing sequences of outbreak and other enterovirus A type 71 (EV-A71) subgenogroup C4 strains isolated in Russia during 2012–2013 with the most closely related sequences in GenBank. Complete viral protein 1 genome regions (891 nt) were compared. The tree was reconstructed by using a coalescent Bayesian algorithm implemented in BEAST 1.7.5 (11) with the SRD06 substitution model, relaxed exponential clock, and constant population prior. The dataset included 63 GenBank sequence

Figure 2. Phylogenetic tree comparing sequences of outbreak and other enterovirus A type 71 (EV-A71) subgenogroup C4 strains isolated in Russia during 2012–2013 with the most closely related sequences in GenBank. Complete viral protein 1 genome regions (891 nt) were compared. The tree was reconstructed by using a coalescent Bayesian algorithm implemented in BEAST 1.7.5 (11) with the SRD06 substitution model, relaxed exponential clock, and constant population prior. The dataset included 63 GenBank sequences that were most similar to the outbreak virus, as determined by using BLAST (http://www.ncbi.nlm.nih.gov/blast/Blast.cgi), and 22 EV-A71 subgenogroup C4 sequences obtained elsewhere in Russia, Kazakhstan, and Kyrgyzstan during 2011–2013 (10). Only the part of the tree that is relevant to the discussion is shown. Strain names contain the GenBank or internal reference number, country of isolation, city code for Russian isolates, and year of isolation. City codes: CEK, Chelyabinsk; CHN, China; KAZ, Kazakhstan; KGZ, Kyrgyzstan; KHV, Khabarovsk; MOW, Moscow; OMS, Omsk; ROV, Rostov; RUS, Russia; STW, Stavropol; SVX, Yekaterinburg; UFA, Ufa. GenBank accession numbers for previously unpublished viruses (KM023173–KM023191‏) are shown in the tree. The dotted line shows the outbreak onset on May 31 (some strains were isolated after that date). Circled letters indicate sequences for viruses isolated in Rostov-on-Don and the Rostov Region of Russia (A); Stavropol region of Russia, which neighbors Rostov Region (B); Siberia, the Pacific region of Russia, Kazakhstan, and Kyrgyzstan (C); and Stavropol, central Russia and Ural Region (D). Scale bar indicates year before the 2013 outbreak.

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