Volume 22, Number 12—December 2016
Letter
Chlamydia-Related Bacteria in Free-Living and Captive Great Apes, Gabon
Table
Analysis of 7 captive and free-living apes for Chlamydia-related bacteria by using real-time PCR and sequencing, Gabon*
Ape | Source | Species | Mean Ct | DNA copies/μL | Closest BLAST† match for 16S rRNA gene | Sequence identity, % | Fragment size, bp | E-value |
---|---|---|---|---|---|---|---|---|
Cola‡ | Rectal swab | Gorilla gorilla gorilla | 33.02 | 10.57 | Waddlia chondrophila WSU 86–1044, complete sequence | 100 | 230 | 1 × 10–115 |
Cabinda‡ | Penile swab | Pan troglodytes troglodytes | 33.32 | 8.58 | W. chondrophila WSU 86–1044, complete sequence | 98 | 241 | 1 × 10–111 |
Djela‡ | Vaginal swab§ | P. troglodytes troglodytes | 29.34 | 122.41 | Candidatus Rhabdochlamydia sp. cvE88, partial sequence | 99 | 243 | 8 × 10–118 |
1882¶ | Feces | P. troglodytes troglodytes | 34.29 | 8.24 | Uncultured Chlamydiales CRG97, partial sequence | 98 | 201 | 6 × 10–93 |
1883¶ | Feces | P. troglodytes troglodytes | 31.90 | 43.55 | Uncultured Chlamydiales CRG97, partial sequence | 99 | 200 | 1 × 10–95 |
1885¶ | Feces | P. troglodytes troglodytes | 31.16 | 73.41 | Uncultured Chlamydiales CRG97, partial sequence | 96 | 209 | 1 × 10–90 |
Gab2130¶ | Feces | G. gorilla gorilla | 35.30 | 2.38 | W. chondrophila WSU 86–1044, complete sequence | 100 | 218 | 5 × 10–109 |
*Ct, cycle threshold.
†http://blast.ncbi.nlm.nih.gov/Blast.cgi
‡Captive ape.
§A second vaginal swab specimen from the same chimpanzee that was collected 1 mo later still showed a positive result by real-time PCR, and sequencing indicated the presence of a Candidatus Rhabdochlamydia sp. cvE88.
¶Free-living ape.
1These authors contributed equally to this article.