Highly Pathogenic Reassortant Avian Influenza A(H5N1) Virus Clade 220.127.116.11a in Poultry, Bhutan
, John Franks1
, Sangay Tenzin, Narapati Dahal, Kinzang Dukpa, Jambay Dorjee, Mohammed M. Feeroz, Jerold E. Rehg, Subrata Barman, Scott Krauss, Pamela McKenzie, Richard Webby, and Robert G. Webster
Author affiliations: St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (A. Marinova-Petkova, J. Franks, J.E. Rehg, S. Barman, S. Krauss, P. McKenzie, R.J. Webby, R.G. Webster); National Centre for Animal Health, Ministry of Agriculture and Forests, Thimphu, Bhutan (S. Tenzin, N. Dahal, K. Dukpa, J. Dorjee); Jahangirnagar University, Dhaka, Bangladesh (M.M. Feeroz)
Figure 2. Pathogenesis of influenza virus rH5N1 and pH5N1 18.104.22.168a genotypes in vitro and in vivo. A) Replication kinetics of rH5N1 and pH5N1 in Madin-Darby canine kidney (mammalian) cells. B) Replication kinetics of rH5N1 and pH5N1 in chicken embryonic fibroblast (avian) cells. C) Oropharyngeal shedding and transmissibility of rH5N1 and pH5N1 in a single-virus transmission model in 5-week-old White Leghorn chickens. D) Cloacal shedding and transmissibility of rH5N1 and pH5N1 in a single-virus transmission model in 5-week-old White Leghorn chickens. Naive chickens were co-housed with donors infected with either Ck/22478 (pH5N1) or Ck/Bh/346 (rH5N1) (C and D). The dashed line in each panel represents the limit of virus detection. ANOVA, analysis of variance; Ck/22478, A/chicken/Bangladesh/22478; Ck/Bh/346, A/chicken/Bhutan/346/2012; Dk/BD/23126, A/duck/Bangladesh/23126; Dk/BD/19097/2013, A/duck/Bangladesh/19097; EID, egg infectious dose; dpi, days postinoculation; hpi, hours postinfection; pH5N1, pandemic H1N1; rH5N1, reassortant H5N1.
Page created: November 18, 2016
Page updated: November 18, 2016
Page reviewed: November 18, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.