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Volume 22, Number 12—December 2016
Dispatch

Highly Pathogenic Reassortant Avian Influenza A(H5N1) Virus Clade 2.3.2.1a in Poultry, Bhutan

Atanaska Marinova-Petkova12, John Franks1, Sangay Tenzin, Narapati Dahal, Kinzang Dukpa, Jambay Dorjee, Mohammed M. Feeroz, Jerold E. Rehg, Subrata Barman, Scott Krauss, Pamela McKenzie, Richard Webby, and Robert G. WebsterComments to Author 
Author affiliations: St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (A. Marinova-Petkova, J. Franks, J.E. Rehg, S. Barman, S. Krauss, P. McKenzie, R.J. Webby, R.G. Webster); National Centre for Animal Health, Ministry of Agriculture and Forests, Thimphu, Bhutan (S. Tenzin, N. Dahal, K. Dukpa, J. Dorjee); Jahangirnagar University, Dhaka, Bangladesh (M.M. Feeroz)

Main Article

Table

Results of hemagglutination inhibition assays of highly pathogenic avian influenza H5N1 viruses isolated in Bhutan, 2012*

Antigens Genetic clade Postinfection ferret antisera
α-A/BHG/QH/IA
clade 2.2 α-A/Hubei/1/2010
clade 2.3.2.1a α-A/ck/BD/15205
clade 2.3.2.1a α-A/dk/BD/19097
clade 2.3.2.1a† α-A/ck/Bhutan/346
clade 2.3.2.1a†
Reference antigens
A/BHG/QH/IA 2.2 320 40 40 80 40
A/Hubei/1/2010 2.3.2.1a 40 640 160 1280 80
A/ck/BD/15205 2.3.2.1a 10 80 80 320 40
A/dk/BD/19097 2.3.2.1a - 40 80 320 40
A/ck/Bhutan/346
 2.3.2.1a
 10
 40
 80
 640
 80
Test antigens
A/chicken/Bhutan/257/2012 2.3.2.1a 20 40 40 640 40
A/chicken/Bhutan/260/2012 2.3.2.1a 20 40 80 640 80
A/wild bird/Bhutan/357/2012 2.3.2.1a 20 40 80 640 40
A/chicken/Bhutan/1026/2012 2.3.2.1a 40 40 80 1280 80
A/chicken/Bhutan/1030/2012 2.3.2.1a 80 160 320 1280 320
A/chicken/Bhutan/317/2012 2.3.2.1a 10 40 80 640 80
A/wild bird/Bhutan/326/2012 2.3.2.1a 10 80 40 320 20
A/wild bird/Bhutan/328/2012 2.3.2.1a 40 20 40 640 80
A/wild bird/Bhutan/356/2012 2.3.2.1a 40 160 160 640 80
A/chicken/Bhutan/406/2012 2.3.2.1a 20 40 80 320 80
A/chicken/Bhutan/413/2012 2.3.2.1a 20 40 80 640 40
A/chicken/Bhutan/505/2012 2.3.2.1a 80 40 80 640 80
A/chicken/Bhutan/933/2012 2.3.2.1a 40 40 80 640 80
GMT (95% CI) 27.54 (18.36–41.30) 49.51 (34.61–70.83) 80 (56.83–112.6) 640 (502.5–815.1) 68.17 (46.24–100.5)

*Boldface indicates homologous titers. A/BHG/QH/IA, A/bar-headed goose/Qinghai/IA/2005; A/ck/BD/15205, A/chicken/Bangladesh/15205/2012; A/ck/Bhutan/346, A/chicken/Bhutan/346/2012; A/dk/BD/19097, A/duck/Bangladesh/19097/2013; GMT, geometric mean titer.
†The immune response in ferrets was boosted with Freund’s incomplete adjuvant (InvivoGen, San Diego, CA, USA) at day 14 postinfection.

Main Article

1These authors contributed equally to this article.

2Current affiliation: Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Page created: November 18, 2016
Page updated: November 18, 2016
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