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Volume 22, Number 2—February 2016
Dispatch

Mediterranean Fin Whales (Balaenoptera physalus) Threatened by Dolphin MorbilliVirus

Sandro MazzariolComments to Author , Cinzia Centelleghe, Giorgia Beffagna, Michele Povinelli, Giuliana Terracciano, Cristiano Cocumelli, Antonio Pintore, Daniele Denurra, Cristina Casalone, Alessandra Pautasso, Cristina Esmeralda Di Francesco, and Giovanni Di Guardo
Author affiliations: University of Padova, Padua, Italy (S. Mazzariol, C. Centelleghe, G. Beffagna, M. Povinelli); Istituto Zooprofilattico Sperimentale del Lazio e della Toscana, Rome, Italy (G. Terracciano, C. Cocumelli); Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy (A. Pintore, D. Denurra); Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Turin, Italy (C. Casalone, A. Pautasso); University of Teramo Faculty of Veterinary Medicine, Teramo, Italy (C.E. Di Francesco, G. Di Guardo)

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Figure 2

Genomic organization of the dolphin morbillivirus (GenBank accession no. KR337460) isolated from a newborn fin whale found stranded on Elba Island, Italy, October 2013. Boxes in first row indicate amino acid changes identified in each gene; asterisks indicate nonsynonymous amino acid substitutions. Boxes in the second row indicate the Morbillivirus gene structure; horizontal lines indicate noncoding sequences. Boxes in the third row indicate the total length of the 2 analyzed virus fragments, an

Figure 2. Genomic organization of the dolphin morbillivirus (GenBank accession no. KR337460) isolated from a newborn fin whale found stranded on Elba Island, Italy, October 2013. Boxes in first row indicate amino acid changes identified in each gene; asterisks indicate nonsynonymous amino acid substitutions. Boxes in the second row indicate the morbillivirus gene structure; horizontal lines indicate noncoding sequences. Boxes in the third row indicate the total length of the 2 analyzed virus fragments, and their sequences are shown, respectively, in boxes in the bottom row. N, nucleoprotein gene; P/V/C, phosphoprotein gene with nonstructural proteins V and C; H, hemagglutinin gene; F, fusion gene; L, large protein gene. Primers DMV-N1 and DMV-P2 (11) were used to amplify a 1,355-bp nt sequence (left box in bottom row) representing partial portion of N gene and P/V/C gene (shown in uppercase letters); lowercase letters indicate noncoding sequences. Three overlapping primer pairs were used to amplify a 1,699-bp nt sequence (right box in bottom row) representing the entire H gene. Underlined bases indicate nucleotide variations from the complete DMV sequence (GenBank accession no. AJ608288). The distribution of amino acid changes identified in each gene are shown in the top row.

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