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Volume 22, Number 2—February 2016
Letter

Mycoplasma pneumoniae Monoclonal P1 Type 2c Outbreak, Russia, 2013

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To the Editor: Mycoplasma pneumoniae is a major cause of respiratory infections among children and young adults and is responsible for up to 40% of all community-acquired pneumonia. In 2011, an epidemic of M. pneumoniae infection was reported in several countries in Europe and Asia and in Israel that primarily involved adhesin P1 type 1 strains and only a few P1 type 2 strains (1,2). The spread of M. pneumoniae was polyclonal (13), except in a few semiclosed settings, such as schools (4). Recently, a new adhesin P1 type 2 variant, named 2c, was reported (5,6) and accounted for 8.3% of 96 M. pneumoniae–positive samples in Germany (7).

In 2013, an increase in the number of community-acquired pneumonia cases was reported in children and their adult contacts from 2 towns in Russia separated by 45 km, Ozerniy and Duchovshina, during January–March and October–November, respectively. To characterize the outbreak in Ozerniy, we collected 13 throat swabs from 9 symptomatic children and 4 asymptomatic adults who were the parents or grandparents of the affected children. All children attended the same school and were treated in the same district hospital as inpatients or outpatients. In Duchovshina, throat swab samples were collected from 17 children and 2 adults. The children attended the same school, and the preschool-aged children visited the same daycare center 1 km away. One adult patient was the first aid driver who transported the children to the hospital. The other adult patient was a community center worker who spent time with the children. In both cities, the symptomatic patients received β-lactams as initial therapy before testing.

All specimens were processed in the laboratory of molecular diagnostics of the Smolensk State Medical Academy (Smolensk Russia). Nucleic acids were extracted by using the DNA-sorb-AM nucleic-acid extraction kit (InterLabService, Moscow, Russia), and M. pneumoniae was subsequently detected by using the AmpliSens Mycoplasma pneumoniae/Chlamydophila pneumoniae-FRT PCR kit (InterLabService). Two M. pneumoniae molecular typing methods, adhesin P1 typing and multilocus variable-number tandem-repeat analysis (MLVA), were performed as previously described (1,5,7). Macrolide resistance-associated mutations were searched using real-time PCR and melting curve analysis (1).

The M. pneumoniae isolates from the specimens collected in Ozerniy were all adhesin P1 type 2c and belonged to 4 distinct MLVA types, 1 of which, MLVA type 73563, has not been previously reported (Table). Without including the instable MPN1 marker (8), we observed only 2 MLVA types. The 19 M. pneumoniae isolates from the specimens collected in Duchovshina also were all P1 type 2c, and all belonged to the same MLVA type, 43562 (type M). No macrolide resistance–associated mutation was observed in any city.

For comparison purposes, because no previous data regarding M. pneumoniae molecular epidemiology in Russia were available, we retrospectively characterized 29 specimens, not from an outbreak, that were previously randomly collected for community-acquired pneumonia etiologic studies during October 2006–October 2007 and February–October 2010 by the laboratory of Smolensk State Medical Academy (Table). Of these specimens, 12 (41%) were P1 type 1, 15 (52%) were P1 type 2a, and only 2 (7%) were P1 type 2c. A polyclonal distribution with 8 distinct MLVA types was observed, with the MLVA type M representing 11 (38%) of the identified MLVA types. Without the MPN1 marker, 3 MLVA types were observed. No macrolide resistance–associated mutation was detected, similar to what was observed in the 32 specimens collected in 2013. This finding is consistent with the low prevalence of macrolide resistance reported in northern Europe (6,7).

We report 2 outbreaks of M. pneumoniae infections that occurred in the first and last quarter of 2013 in western Russia (Smolensk region). Despite the high predominance of P1 type 1 strains reported in the recent literature (1,2,7), these 2 outbreaks, reported in semiclosed settings involved only the newly described P1 type 2c variant; 1 outbreak represented a monoclonal phenomenon. In the Smolensk region, the circulation of both type 1 and 2 strains was observed a few years before the outbreak; most of these strains were P1 type 2a variants, and only a minority were type 2c variants, suggesting that the new type 2c variant had spread throughout this region of Russia since at least 2006. In other parts of the world, a switch between type 1 and type 2 strains might be occurring. Indeed, in the United States, P1 type 1 isolates predominated before 2010 but dropped to 50% of isolates in 2013, and type 2 and type 2 variant strains increased (9). This cyclic pattern of type 1 or type 2 predominance in the population has previously been reported (10).

In conclusion, we detected no macrolide resistance in western Russia. The P1 type 2c variant spread throughout this region and can be responsible for monoclonal outbreaks. The epidemiologic monitoring of M. pneumoniae P1 types will assess the potential switch to P1 type 2 in the United States and other parts of the world and detect the possible emergence of the P1 type 2c variant.

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Acknowledgment

This study was supported by internal funding.

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Inna Edelstein, Svetlana Rachina, Arabella Touati, Roman Kozlov, Nadège Henin, Cécile Bébéar, and Sabine PereyreComments to Author 

Author affiliations: Smolensk State Medical University of Ministry of Health of Russian Federation, Smolensk, Russian Federation (I. Edelstein, R. Kozlov); Inter-regional Association for Clinical Microbiology & Antimicrobial Chemotherapy, Smolensk (S. Rachina); University of Bordeaux, Bordeaux, France (A. Touati, N. Henin, C. Bébéar, S. Pereyre); Institut National de la Recherche Agronomique, Bordeaux (A. Touati, N. Henin, C. Bébéar, S. Pereyre); Bordeaux University Hospital, Bordeaux (C. Bébéar, S. Pereyre)

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References

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  2. Liu  Y, Ye  X, Zhang  H, Xu  X, Wang  M. Multiclonal origin of macrolide-resistant Mycoplasma pneumoniae isolates as determined by multilocus variable-number tandem-repeat analysis. J Clin Microbiol. 2012;50:27935. DOIPubMed
  3. Chalker  V, Stocki  T, Litt  D, Bermingham  A, Watson  J, Fleming  D, Increased detection of Mycoplasma pneumoniae infection in children in England and Wales, October 2011 to January 2012. Euro Surveill. 2012;17:20081 .PubMed
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  6. Spuesens  EB, Hoogenboezem  T, Sluijter  M, Hartwig  NG, van Rossum  AM, Vink  C. Macrolide resistance determination and molecular typing of Mycoplasma pneumoniae by pyrosequencing. J Microbiol Methods. 2010;82:21422. DOIPubMed
  7. Dumke  R, Schnee  C, Pletz  MW, Rupp  J, Jacobs  E, Sachse  K, Mycoplasma pneumoniae and Chlamydia spp. infection in community-acquired pneumonia, Germany, 2011–2012. Emerg Infect Dis. 2015;21:42634. DOIPubMed
  8. Chalker  VJ, Pereyre  S, Dumke  R, Winchell  J, Khosla  P, Sun  H, International Mycoplasma pneumoniae typing study: interpretation of M. pneumoniae multilocus variable-number tandem-repeat analysis. New Microbes New Infect. 2015;7:37–40.
  9. Diaz  MH, Benitez  AJ, Winchell  JM. Investigations of Mycoplasma pneumoniae infections in the United States: trends in molecular typing and macrolide resistance from 2006 to 2013. J Clin Microbiol. 2015;53:12430. DOIPubMed
  10. Kenri  T, Okazaki  N, Yamazaki  T, Narita  M, Izumikawa  K, Matsuoka  M, Genotyping analysis of Mycoplasma pneumoniae clinical strains in Japan between 1995 and 2005: type shift phenomenon of M. pneumoniae clinical strains. J Med Microbiol. 2008;57:46975 . DOIPubMed

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Cite This Article

DOI: 10.3201/eid2202.151349

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Table of Contents – Volume 22, Number 2—February 2016

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Sabine Pereyre, USC EA3671 Mycoplasmal and Chlamydial Infections in Humans, University of Bordeaux, Campus Bordeaux Carreire, 146 rue Léo Saignat, 33076 Bordeaux, France

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Page created: January 19, 2016
Page updated: January 19, 2016
Page reviewed: January 19, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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