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Volume 22, Number 3—March 2016
Research

Underestimation of Invasive Meningococcal Disease in Italy

Chiara Azzari1Comments to Author , Francesco Nieddu1, Maria Moriondo1, Giuseppe Indolfi, Clementina Canessa, Silvia Ricci, Leila Bianchi, Daniele Serranti, Giovanni Maria Poggi, and Massimo Resti
Author affiliations: Anna Meyer Children’s University Hospital, Florence (C. Azzari, F. Nieddu, M. Moriondo, G. Indolfi, C. Canessa, S. Ricci, L. Bianchi, D. Serranti, G.M. Poggi, M. Resti); University of Florence Department of Health Sciences, Florence, Italy (C. Azzari, F. Nieddu, M. Moriondo, S. Ricci, D. Serranti, G.M. Poggi)

Main Article

Table 1

Primers and probes used for Neisseria meningitidis serogrouping of isolates from samples from a national register for molecular surveillance of invasive bacterial disease, Italy, 2007–2014

Target
 Gene
 Forward primer
 Reverse primer
 Probe
N. meningitidis ctrA gctgcggtaggtggttcaa ttgtcgcggatttgcaacta FAM_cattgccacgtgtcagctgcacat_TAMRA
Serogroup A sacB cccccagcatggctagattt agggcactttgtggcataattt FAM_accctaaaattcaatgggtatatcacga_TAMRA
Serogroup B siaD B ttggacttggttaagctgacctaa gttgacaacatctccattttatcttacc FAM_ttagatatgacaaataaattgttacgtggg_TAMRA
Serogroup C siaD C agggaaccgcaacctatgc cacaaaacgttgctcaaattttg FAM_ccactcttagaatcatttacatacaaaccc_TAMRA
Serogroup W/Y siaD W/Y gctgataaattgttcttatggtctgaa cggcaccagaaccaatctct FAM_ttggaatcatgagcttttaccaaatccaaca_TAMRA
Serogroup W* siaD cagaagtgagggatttccata cacaaccattttcattatagttactgt
Serogroup Y* siaD ctcaaagcgaaggctttggtta ctgaagcgttttcattataattgctaa

*Identified by using endpoint PCR.

Main Article

1These authors contributed equally to this article.

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Page updated: February 18, 2016
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