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Volume 22, Number 5—May 2016
CME ACTIVITY - Research

Spectrum of Viral Pathogens in Blood of Malaria-Free Ill Travelers Returning to Canada

Ruwandi Kariyawasam, Rachel Lau, Alireza Eshaghi, Samir N. Patel, Doug Sider, Jonathan B. Gubbay, and Andrea K. BoggildComments to Author 
Author affiliations: Public Health Ontario Laboratories, Toronto, Ontario, Canada (R. Kariyawasam, R. Lau, A. Eshaghi, S.N. Patel, J.B. Gubbay, A.K. Boggild); University of Toronto, Toronto (S.N. Patel, J.B. Gubbay, A.K. Boggild); Public Health Ontario, Toronto (D. Sider); McMaster University, Hamilton, Ontario, Canada (D. Sider); Toronto General Hospital, Toronto (A.K. Boggild)

Main Article

Table 1

Primers and probes used to test specimens from ill travelers returning to Canada for viral DNA*

Primer and probe Sequence, 5′ → 3′ Concentration, nmol/L Reference
Human B2MG (20)
B2MG fwd primer TGAGTATGCCTGCCGTGTGA 900
B2MG rev primer ACTCATACACAACTTTCAGCAGCTTAC 900
B2MG probe
 HEX-CCATGTGACTTTGTCACAGCCCAAGATAGTT-BHQ1
 200
Plasmodium (21)
Plasmo1 primer GTTAAGGGAGTGAAGACGATCAGA 200
Plasmo2 primer AACCCAAAGACTTTGATTTCTCATAA 200
Plasmo probe
 FAM-ACCGTCGTAATCTTAACCATAAACTATGCCGACTAG-BHQ1
 50
HSV-1 and -2 (22)
GbTypF primer CGCATCAAGACCACCTCCTC 600
GbTypR primer GCTCGCACCACGCGA 600
GbTyp1 probe VIC-TGGCAACGCGGCCCAAC-TAMRA 200
GbTyp2probe
 FAM-CGGCGATGCGCCCCAG-TAMRA
 200
CMV (23)
CMV IE fwd primer GACTAGTGTGATGCTGGCCAAG 500
CMV IE rev primer GCTACAATAGCCTCTTCCTCATCTG 500
CMV IE probe
 HEX-AGCCTGAGGTTATCAGTGTAATGAAGCGCC-BHQ1
 125
EBV (23)
EBV-BALF fwd primer CGGAAGCCCTCTGGACTTC 500
EBV-BALF5 rev primer CCCTGTTTATCCGATGGAATG 500
EBV BALF5 probe FAM-TGTACACGCACGAGAAATGCGCC-BHQ1 125

*B2MG, β2 microglobulin; CMV, cytomegalovirus; EBV, Epstein-Barr virus; fwd, forward; HSV, herpes simplex virus; rev, reverse.

Main Article

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Page updated: April 02, 2018
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