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Volume 22, Number 5—May 2016
CME ACTIVITY - Research

Spectrum of Viral Pathogens in Blood of Malaria-Free Ill Travelers Returning to Canada

Ruwandi Kariyawasam, Rachel Lau, Alireza Eshaghi, Samir N. Patel, Doug Sider, Jonathan B. Gubbay, and Andrea K. BoggildComments to Author 
Author affiliations: Public Health Ontario Laboratories, Toronto, Ontario, Canada (R. Kariyawasam, R. Lau, A. Eshaghi, S.N. Patel, J.B. Gubbay, A.K. Boggild); University of Toronto, Toronto (S.N. Patel, J.B. Gubbay, A.K. Boggild); Public Health Ontario, Toronto (D. Sider); McMaster University, Hamilton, Ontario, Canada (D. Sider); Toronto General Hospital, Toronto (A.K. Boggild)

Main Article

Table 2

Primers and probes used to test specimens from ill travelers returning to Canada for viral RNA*

Primer and probe Sequence, 5′→ 3′ Concentration, nmol/L Reference
Pandengue-MS2 (23,24)
Den Eili fwd primer GGACTAGAGGTTAGAGGAGACCCC 500
Den Eili rev primer GAGACAGCAGGATCTCTGGTC 500
Den Eili probe FAM-AGCATATTGACGCTGGGA-MGB-BHQ1 125
MS2-TM2 fwd primer TGCTCGCGGATACCCG 500
MS2-TM2 rev primer AACTTGCGTTCTCGAGCGAT 500
MS2-TM2 probe
 Quasar670-ACCTCGGGTTTCCGTCTTGCTCGT-BHQ1
 125
Dengue virus serotyping (25)
DENV-1 fwd primer CAAAAGGAAGTCGTGCAATA 500
DENV-1 rev primer CTGAGTGAATTCTCTCTACTGAACC 500
DENV-1 probe FAM-CATGTGGTTGGGAGCACGC-BHQ1 125
DENV-2 fwd primer CAGGTTATGGCACTGTCACGAT 500
DENV-2 rev primre CCATCTGCAGCAACACCATCTC 500
DENV-2 probe HEX-CTCTCCGAGAACAGGCCTCGACTTCAA-BHQ-1 125
DENV-3 fwd primer GGACTGGACACACGCACTCA 500
DENV-3 rev primer CATGTCTCTACCTTCTCGACTTGTCT 500
DENV-3 probe FAM-ACCTGGATGTCGGCTGAAGGAGCTTG-BHQ1 125
DENV-4 fwd primer TTGTCCTAATGATGCTGGTCG 500
DENV-4 rev primer TCCACCTGAGACTCCTTCCA 500
DENV-4 probe
 HEX-TTCCTACTCCTACGCATCGCATTCCG-BHQ1
 125
Panflavivirus (26)
Flavi all S (sense) TACAACATgATggggAARAgAgARAA 500
Flavi all AS2 (antisense) gTgTCCCAgCCNgCKgTgTCATCWgC 500
DENV-4F TACAACATgATgggRAAACgTgAGAA 500
Flavi all probe 1 FAM-AARggHAgYMgNgCCA+TH+T+g+g+T-BBQ† 100
Flavi probe 3a FAM-CCgTgCCATATggTATATgTggCTgggAgC-BBQ† 100
Flavi probe 3b
 FAM-TTTCTggAATTTgAAgCCCTgggTTT-BBQc
 100
Hepatitis A virus (27); vital SOP‡
HAV 68 TCACCGCCGTTTGCC 500
HAV240 GGAGAGCCCTGGAAGAAAG 500
HAV150 probe
 FAM-CCTGAACCTGCAGGAATTAA-MGB-NFQ
 125
Chikungunya virus (28)
ChikF10378–10398 GCATCAGCTAAGCTCCGGGTC 500
ChikR 10487–10508¶ GGTGTCCAGGCTGAAGACATTG 500
Chik Pongsiri HEX-ATGCAAACGGCGACCATGCCGTCA-VIC 125

*The primers and probes were used with the Applied Biosystems 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA). fwd, forward; rev, reverse; SOP, standard operating procedure.
†Locked nucleic acid bases.
‡Vital Standard Operating Procedure modified from Costafreda et al. (27).
¶ChikR10487-10508 reverse complement used from published sequence.

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Page updated: April 02, 2018
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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