Volume 22, Number 8—August 2016
Synopsis
Co-infections in Visceral Pentastomiasis, Democratic Republic of the Congo
Dennis Tappe
, Mihály Sulyok, Therese Riu, Lajos Rózsa, Imre Bodó, Christoph Schoen, Birgit Muntau, Gergely Babocsay, and Richard Hardi
, Mihály Sulyok, Therese Riu, Lajos Rózsa, Imre Bodó, Christoph Schoen, Birgit Muntau, Gergely Babocsay, and Richard Hardi
Table
Patient characteristics and sequencing results of pentastomid 18S rDNA and Armillifer-specific cytochrome oxidase gene PCRs, Sankuru District, Democratic Republic of the Congo, 2014–2015*
| Patient no. | Age, y/sex | Type of surgery | Location of pentastomid cyst | Sequencing result |
Histology | |
|---|---|---|---|---|---|---|
| Pentastomid 18S rDNA-gene, 430 bp | Armillifer cytochrome oxidase subunit I-gene, 288 bp | |||||
| 1† |
NA/M |
Laparotomy |
Peritoneum |
Cyst 1: A. grandis | A. grandis | Not done |
| Cyst 2: negative | A. armillatus | Not done | ||||
| Cyst 3: Raillietiella sp.‡ | Negative‡ | Necrotic pentastomid | ||||
| Cyst 4: negative§ |
Negative§ |
Necrotic pentastomid |
||||
| 2† |
35/F |
Laparotomy |
Peritoneum |
Cyst 1: A. grandis | A. grandis | Not done |
| Cyst 2: A. armillatus | A. armillatus | Not done | ||||
| Cyst 3: negative‡ | A. grandis‡ | Necrotic pentastomid | ||||
| Cyst 4: negative‡ | A. grandis‡ | Total necrosis | ||||
| Cyst 5: negative‡ | A. grandis‡ | Necrotic pentastomid | ||||
| Cyst 6: negative‡ |
A. grandis‡ |
Total necrosis |
||||
| 3† |
NA/M |
Laparotomy |
Peritoneum |
Cyst 1: A. grandis | A. grandis | Not done |
| Cyst 2: A. armillatus | A. armillatus | Not done. | ||||
| Cyst 3: A. grandis | Negative | Not done | ||||
| Cyst 4: negative§ | A. grandis§ | Total necrosis | ||||
| Cyst 5: negative§ | A. armillatus§ | Total necrosis | ||||
| Cyst 6: negative‡ |
A. armillatus§ |
Total necrosis |
||||
| 4 |
NA/M |
Hernioplasty |
Peritoneum |
Cyst 1: A. armillatus |
A. armillatus |
Not done |
| 5 |
NA/F |
Cesarean section |
Omentum |
Cyst 1: A. armillatus | A. armillatus | Not done |
| Cyst 2: A. armillatus‡ |
A. armillatus‡ |
Necrotic pentastomid |
||||
| 6 |
36/F |
Laparotomy, appendectomy |
Left ovary |
Cyst 1: A. armillatus | A. armillatus | Not done |
| Cyst 2: not done | Not done | Ovarian cyst | ||||
| Cyst 3: not done |
Not done |
Ovarian cyst |
||||
| 7 | 42/F | Laparotomy, appendectomy | Omentum | Cyst 1: A. armillatus | A. armillatus | Not done |
*NA, not available; negative, PCR negative or no positive sequence result.
†Co-infected.
‡PCRs performed from paraffin block tissue slices.
§Repeat PCRs performed from whole paraffin block, after PCRs from block slices were negative. Tissue materials from patient 1 (cysts 1, 2), patient 3 (cyst 3), and patient 7 (cyst 1) were ground, as the tissue was too hard to cut into pieces for PCR.
Page created: July 15, 2016
Page updated: July 15, 2016
Page reviewed: July 15, 2016
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