Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 22, Number 9—September 2016
Research

Staphylococcus aureus Regulatory RNAs as Potential Biomarkers for Bloodstream Infections

Valérie Bordeau, Anne Cady, Matthieu Revest, Octavie Rostan, Mohamed Sassi, Pierre Tattevin, Pierre-Yves Donnio, and Brice FeldenComments to Author 
Author affiliations: University of Rennes 1, Rennes, France (V. Bordeau, A. Cady, M. Revest, O. Rostan, M. Sassi, P. Tattevin, P.-Y. Donnio, B. Felden); Institut National de la Santé et de la Recherche Médicale Unité 835, Rennes (V. Bordeau, M. Sassi, P. Tattevin, B. Felden); Rennes University Hospital, Rennes (A. Cady, M. Revest, P. Tattevin, P.-Y. Donnio)

Main Article

Figure 4

Discrimination of colonizing strains of Staphylococcus aureus from patients with bloodstream infections, Rennes, France. A) RNAIII analysis of strains from colonizing infections and bloodstream infections; B) RNAIII analysis of strains from colonizing infections and persons with septic shock; C) RNAII/sprD analysis of strains from colonizing infections and bloodstream infections; D) RNAII/sprD analysis of strains from colonizing infections and persons with septic shock. RNAIII and sprD levels we

Figure 4. Discrimination of colonizing strains of Staphylococcus aureus from patients with bloodstream infections, Rennes, France. A) RNAIII analysis of strains from carriers and infected persons; B) RNAIII analysis of strains from carriers and persons with nonsevere sepsis or septic shock; C) RNAII/sprD analysis of strains from carriers and infected persons; D) RNAII/sprD analysis of strains from carriers and persons with nonsevere sepsis or septic shock. RNAIII and sprD levels were calculated relative to those for tmRNA. RNAIII expression was monitored by quantitative PCR during early exponential growth phase for 61 strains. p values (by Mann-Whitney U test) for significant differences are shown. Panel C inset shows receiver operating characteristic analysis showing discrimination of carriage strains from infectious strains. Horizontal lines indicate medians. Using the comparative cycle threshold method, we normalized the amount of RNAIII against that of tmRNA relative to that for control strain L102 (methicillin-susceptible S. aureus colonization strain). Each symbol indicates mean for 3 independent experiments. AU, arbitrary units; spr, small pathogenicity island RNA; tmRNA, transfer–messenger RNA.

Main Article

Page created: August 23, 2016
Page updated: August 23, 2016
Page reviewed: August 23, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external