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Volume 23, Number 10—October 2017
Dispatch

Molecular Tracing to Find Source of Protracted Invasive Listeriosis Outbreak, Southern Germany, 2012–2016

Sylvia KletaComments to Author , Jens Andre Hammerl, Ralf Dieckmann, Burkhard Malorny, Maria Borowiak, Sven Halbedel, Rita Prager, Eva Trost, Antje Flieger, Hendrik Wilking, Sabine Vygen-Bonnet, Ulrich Busch, Ute Messelhäußer, Sabine Horlacher, Katharina Schönberger, Dorothee Lohr, Elisabeth Aichinger, Petra Luber, Andreas Hensel, and Sascha Al Dahouk
Author affiliations: German Federal Institute for Risk Assessment, Berlin, Germany (S. Kleta, J.A. Hammerl, R. Dieckmann, B. Malorny, M. Borowiak, A. Hensel, S. Al Dahouk); Robert Koch Institute, Berlin (H. Wilking, S. Vygen-Bonnet); Robert Koch Institute, Wernigerode, Germany (S. Halbedel, R. Prager, E. Trost, A. Flieger); Bavarian Health and Food Safety Authority, Oberschleißheim, Germany (U. Busch, U. Messelhäußer, K. Schönberger); Chemical and Veterinary Investigatory Office, Stuttgart, Germany (S. Horlacher); Baden-Württemberg State Health Office, Stuttgart (D. Lohr, E. Aichinger); Federal Office of Consumer Protection and Food Safety, Berlin (P. Luber)

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Figure 1

ApaI restriction enzyme analysis of Listeria monocytogenes outbreak strain and isolates with >90% similarity to the outbreak strain, southern Germany, 2012–2016. We performed molecular subtyping in line with the PulseNet standardized PFGE protocol for L. monocytogenes (8) and the standard operating procedures of the European Union Reference Laboratory for L. monocytogenes (9) to ensure interlaboratory comparability of the results. We analyzed PFGE patterns using BioNumerics software version 7

Figure 1. ApaI restriction enzyme analysis of Listeria monocytogenes outbreak strain and isolates with >90% similarity to the outbreak strain, southern Germany, 2012–2016. We performed molecular subtyping in line with the PulseNet standardized PFGE protocol for L. monocytogenes (8) and the standard operating procedures of the European Union Reference Laboratory for L. monocytogenes (9) to ensure interlaboratory comparability of the results. We analyzed PFGE patterns using BioNumerics software version 7.5 (Applied Maths, Sint-Martens-Latem, Belgium). Dendrogram indicates percentage similarity between the ApaI PFGE pattern of the outbreak strain and that of the other closely related isolates. Outbreak strain PFGE pattern is labeled with the number 54. PFGE, pulsed-field gel electrophoresis.

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References
  1. Farber  JM, Peterkin  PI. Listeria monocytogenes, a food-borne pathogen. Microbiol Rev. 1991;55:476–511.PubMedGoogle Scholar
  2. Schuchat  A, Swaminathan  B, Broome  CV. Epidemiology of human listeriosis. Clin Microbiol Rev. 1991;4:169–83. DOIPubMedGoogle Scholar
  3. Robert Koch-Institute. Infektionsepidemiologisches Jahrbuch meldepflichtiger Krankheiten für 2016. Berlin: The Institute; 2017 [cited 2017 Mar 1]. http://www.rki.de/jahrbuch
  4. Preußel  K, Milde-Busch  A, Schmich  P, Wetzstein  M, Stark  K, Werber  D. Risk factors for sporadic non-pregnancy associated listeriosis in Germany—immunocompromised patients and frequently consumed ready-to-eat products. PLoS One. 2015;10:e0142986. DOIPubMedGoogle Scholar
  5. Werber  D, Hille  K, Frank  C, Dehnert  M, Altmann  D, Müller-Nordhorn  J, et al. Years of potential life lost for six major enteric pathogens, Germany, 2004-2008. Epidemiol Infect. 2013;141:961–8. DOIPubMedGoogle Scholar
  6. Ruppitsch  W, Prager  R, Halbedel  S, Hyden  P, Pietzka  A, Huhulescu  S, et al. Ongoing outbreak of invasive listeriosis, Germany, 2012 to 2015. Euro Surveill. 2015;20:pii=30094. DOIPubMedGoogle Scholar
  7. Ruppitsch  W, Pietzka  A, Prior  K, Bletz  S, Fernandez  HL, Allerberger  F, et al. Defining and evaluating a core genome multilocus sequence typing scheme for whole-genome sequence-based typing of Listeria monocytogenes. J Clin Microbiol. 2015;53:2869–76. DOIPubMedGoogle Scholar
  8. Halpin  JL, Garrett  NM, Ribot  EM, Graves  LM, Cooper  KL. Re-evaluation, optimization, and multilaboratory validation of the PulseNet-standardized pulsed-field gel electrophoresis protocol for Listeria monocytogenes. Foodborne Pathog Dis. 2010;7:293–8. DOIPubMedGoogle Scholar
  9. Félix  B, Dao  TT, Lombard  B, Asséré  A, Brisabois  A, Roussel  S. The use of pulsed field gel electrophoresis in Listeria monocytogenes sub-typing—harmonization at the European Union level. In: Magdeldin S, editor. Gel electrophoresis—principles and basics. Rijeka (Croatia): In Tech; 2012. p. 241–54.

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