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Volume 23, Number 10—October 2017

Dispatch

Molecular Tracing to Find Source of Protracted Invasive Listeriosis Outbreak, Southern Germany, 2012–2016

Sylvia KletaComments to Author , Jens Andre Hammerl, Ralf Dieckmann, Burkhard Malorny, Maria Borowiak, Sven Halbedel, Rita Prager, Eva Trost, Antje Flieger, Hendrik Wilking, Sabine Vygen-Bonnet, Ulrich Busch, Ute Messelhäußer, Sabine Horlacher, Katharina Schönberger, Dorothee Lohr, Elisabeth Aichinger, Petra Luber, Andreas Hensel, and Sascha Al Dahouk
Author affiliations: German Federal Institute for Risk Assessment, Berlin, Germany (S. Kleta, J.A. Hammerl, R. Dieckmann, B. Malorny, M. Borowiak, A. Hensel, S. Al Dahouk); Robert Koch Institute, Berlin (H. Wilking, S. Vygen-Bonnet); Robert Koch Institute, Wernigerode, Germany (S. Halbedel, R. Prager, E. Trost, A. Flieger); Bavarian Health and Food Safety Authority, Oberschleißheim, Germany (U. Busch, U. Messelhäußer, K. Schönberger); Chemical and Veterinary Investigatory Office, Stuttgart, Germany (S. Horlacher); Baden-Württemberg State Health Office, Stuttgart (D. Lohr, E. Aichinger); Federal Office of Consumer Protection and Food Safety, Berlin (P. Luber)

Main Article

Figure 1

ApaI restriction enzyme analysis of Listeria monocytogenes outbreak strain and isolates with >90% similarity to the outbreak strain, southern Germany, 2012–2016. We performed molecular subtyping in line with the PulseNet standardized PFGE protocol for L. monocytogenes (8) and the standard operating procedures of the European Union Reference Laboratory for L. monocytogenes (9) to ensure interlaboratory comparability of the results. We analyzed PFGE patterns using BioNumerics software version 7

Figure 1. ApaI restriction enzyme analysis of Listeria monocytogenes outbreak strain and isolates with >90% similarity to the outbreak strain, southern Germany, 2012–2016. We performed molecular subtyping in line with the PulseNet standardized PFGE protocol for L. monocytogenes (8) and the standard operating procedures of the European Union Reference Laboratory for L. monocytogenes (9) to ensure interlaboratory comparability of the results. We analyzed PFGE patterns using BioNumerics software version 7.5 (Applied Maths, Sint-Martens-Latem, Belgium). Dendrogram indicates percentage similarity between the ApaI PFGE pattern of the outbreak strain and that of the other closely related isolates. Outbreak strain PFGE pattern is labeled with the number 54. PFGE, pulsed-field gel electrophoresis.

Main Article

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