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Volume 23, Number 10—October 2017
Dispatch

Molecular Tracing to Find Source of Protracted Invasive Listeriosis Outbreak, Southern Germany, 2012–2016

Sylvia KletaComments to Author , Jens Andre Hammerl, Ralf Dieckmann, Burkhard Malorny, Maria Borowiak, Sven Halbedel, Rita Prager, Eva Trost, Antje Flieger, Hendrik Wilking, Sabine Vygen-Bonnet, Ulrich Busch, Ute Messelhäußer, Sabine Horlacher, Katharina Schönberger, Dorothee Lohr, Elisabeth Aichinger, Petra Luber, Andreas Hensel, and Sascha Al Dahouk
Author affiliations: German Federal Institute for Risk Assessment, Berlin, Germany (S. Kleta, J.A. Hammerl, R. Dieckmann, B. Malorny, M. Borowiak, A. Hensel, S. Al Dahouk); Robert Koch Institute, Berlin (H. Wilking, S. Vygen-Bonnet); Robert Koch Institute, Wernigerode, Germany (S. Halbedel, R. Prager, E. Trost, A. Flieger); Bavarian Health and Food Safety Authority, Oberschleißheim, Germany (U. Busch, U. Messelhäußer, K. Schönberger); Chemical and Veterinary Investigatory Office, Stuttgart, Germany (S. Horlacher); Baden-Württemberg State Health Office, Stuttgart (D. Lohr, E. Aichinger); Federal Office of Consumer Protection and Food Safety, Berlin (P. Luber)

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Figure 2

Minimum spanning tree estimating the phylogenetic relationships among outbreak and nonoutbreak Listeria monocytogenes isolates from humans and from food products, southern Germany, 2012–2016. We conducted bioinformatics analyses using the Ridom SeqSphere+ software version 3.1.0-2016-01 (Ridom GmbH, Münster, Germany). The core-genome multilocus sequence typing scheme for whole-genome sequencing–based typing of L. monocytogenes relies on a set of 1,701 target genes (alleles) that are present in &g

Figure 2. Minimum spanning tree estimating the phylogenetic relationships among outbreak and nonoutbreak Listeria monocytogenes isolates from humans and from food products, southern Germany, 2012–2016. We conducted bioinformatics analyses using the Ridom SeqSphere+ software version 3.1.0-2016-01 (Ridom GmbH, Münster, Germany). The core-genome multilocus sequence typing scheme for whole-genome sequencing–based typing of L. monocytogenes relies on a set of 1,701 target genes (alleles) that are present in >99% of the known genomes of the species (7). Each circle represents an allelic profile. The numbers on the connecting lines illustrate the number of differing alleles in a pairwise comparison. Closely related genotypes (<10 allele difference) designated a cluster type and are indicated with bold red numbers. CT, cluster type; PFGE, pulsed-field gel electrophoresis.

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