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Volume 23, Number 4—April 2017
Dispatch

Antiviral Drug–Resistant Influenza B Viruses Carrying H134N Substitution in Neuraminidase, Laos, February 2016

Tatiana Baranovich, Phengta Vongphrachanh, Pakapak Ketmayoon, Thongchanh Sisouk, Khampheng Chomlasack, Viengphone Khanthamaly, Ha Thuy Nguyen, Vasiliy P. Mishin, Henju Marjuki, John Barnes, Rebecca J. Garten, James Stevens, David Wentworth, and Larisa GubarevaComments to Author 
Author affiliations: Carter Consulting, Inc., Atlanta, Georgia, USA (T. Baranovich); World Health Organization Collaborating Center for Surveillance, Epidemiology and Control of Influenza, Atlanta (T. Baranovich, V. Khanthamaly, H.T. Nguyen, V.P. Mishin, H. Marjuki, J.R. Barnes, R.J. Garten, J. Stevens, D.E. Wentworth, L.V. Gubareva); Centers for Disease Control and Prevention, Atlanta (T. Baranovich, V. Khanthamaly, H.T. Nguyen, V.P. Mishin, H. Marjuki, J.R. Barnes, R.J. Garten, J. Stevens, D.E. Wentworth, L.V. Gubareva); National Center for Laboratory and Epidemiology, Vientiane, Laos (P. Vongphrachanh, P. Ketmayoon, T. Sisouk, K. Chomlasack); World Health Organization Emerging Disease Surveillance and Response Unit, Vientiane, Laos (P. Ketmayoon); Battelle Memorial Institute, Atlanta (H.T. Nguyen)

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Figure 2

Characterization of influenza B viruses detected in Laos, February 2016. A) Thermostability of neuraminidase (NA) determined after viruses were incubated for 15 min at 4°C or at 30°C–57°C. NA enzyme activity was determined by a fluorescence-based assay (4). B) Replication kinetics of influenza B viruses in fully differentiated human primary NHBE cells that were inoculated with the designated viruses (multiplicity of infection 0.001). Apical washes were taken at indicated times after inoculation,

Figure 2. Characterization of influenza B viruses detected in Laos, February 2016. A) Thermostability of neuraminidase (NA) determined after viruses were incubated for 15 min at 4°C or at 30°C–57°C. NA enzyme activity was determined by a fluorescence-based assay (4). B) Replication kinetics of influenza B viruses in fully differentiated human primary NHBE cells that were inoculated with the designated viruses (multiplicity of infection 0.001). Apical washes were taken at indicated times after inoculation, and virus titers were determined on MDCK cells. The area under the virus titer curve from 2 to 72 h after inoculation (AUC2–72) was determined and compared with that of the control virus by repeated-measures analysis of variance with the Dunnett posttest, using GraphPad Prism 5 software (GraphPad Software, La Jolla, CA, USA). Dashed line represents the limit of detection of the assay (1.75 log10 50% tissue culture infectious dose [TCID50/mL]). Values shown are means and SDs from 2 independent experiments performed in duplicates (n = 4). Error bars represent SDs. NS, not significant.

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References
  1. Burnham  AJ, Baranovich  T, Govorkova  EA. Neuraminidase inhibitors for influenza B virus infection: efficacy and resistance. Antiviral Res. 2013;100:520–34. DOIPubMedGoogle Scholar
  2. Budd  A, Blanton  L, Kniss  K, Smith  S, Mustaquim  D, Davlin  SL, et al. Update: influenza activity—United States and worldwide, May 22–September 10, 2016. MMWR Morb Mortal Wkly Rep. 2016;65:1008–14. DOIPubMedGoogle Scholar
  3. Davlin  SL, Blanton  L, Kniss  K, Mustaquim  D, Smith  S, Kramer  N, et al. Influenza activity—United States, 2015–16 season and composition of the 2016–17 influenza vaccine. MMWR Morb Mortal Wkly Rep. 2016;65:567–75. DOIPubMedGoogle Scholar
  4. Okomo-Adhiambo  M, Mishin  VP, Sleeman  K, Saguar  E, Guevara  H, Reisdorf  E, et al. Standardizing the influenza neuraminidase inhibition assay among United States public health laboratories conducting virological surveillance. Antiviral Res. 2016;128:28–35. DOIPubMedGoogle Scholar
  5. World Health Organization. Meetings of the WHO working group on surveillance of influenza antiviral susceptibility – Geneva, November 2011 and June 2012. Wkly Epidemiol Rec. 2012;87:369–74.PubMedGoogle Scholar
  6. Colman  PM, Hoyne  PA, Lawrence  MC. Sequence and structure alignment of paramyxovirus hemagglutinin-neuraminidase with influenza virus neuraminidase. J Virol. 1993;67:2972–80.PubMedGoogle Scholar
  7. Kawai  N, Ikematsu  H, Iwaki  N, Maeda  T, Satoh  I, Hirotsu  N, et al. A comparison of the effectiveness of oseltamivir for the treatment of influenza A and influenza B: a Japanese multicenter study of the 2003-2004 and 2004-2005 influenza seasons. Clin Infect Dis. 2006;43:439–44. DOIPubMedGoogle Scholar
  8. Monto  AS, McKimm-Breschkin  JL, Macken  C, Hampson  AW, Hay  A, Klimov  A, et al. Detection of influenza viruses resistant to neuraminidase inhibitors in global surveillance during the first 3 years of their use. Antimicrob Agents Chemother. 2006;50:2395–402. DOIPubMedGoogle Scholar
  9. Sugaya  N, Mitamura  K, Yamazaki  M, Tamura  D, Ichikawa  M, Kimura  K, et al. Lower clinical effectiveness of oseltamivir against influenza B contrasted with influenza A infection in children. Clin Infect Dis. 2007;44:197–202. DOIPubMedGoogle Scholar
  10. Sheu  TG, Deyde  VM, Garten  RJ, Klimov  AI, Gubareva  LV. Detection of antiviral resistance and genetic lineage markers in influenza B virus neuraminidase using pyrosequencing. Antiviral Res. 2010;85:354–60. DOIPubMedGoogle Scholar
  11. Takashita  E, Meijer  A, Lackenby  A, Gubareva  L, Rebelo-de-Andrade  H, Besselaar  T, et al. Global update on the susceptibility of human influenza viruses to neuraminidase inhibitors, 2013-2014. Antiviral Res. 2015;117:27–38. DOIPubMedGoogle Scholar
  12. Little  K, Leang  SK, Butler  J, Baas  C, Harrower  B, Mosse  J, et al. Zanamivir-resistant influenza viruses with Q136K or Q136R neuraminidase residue mutations can arise during MDCK cell culture creating challenges for antiviral susceptibility monitoring. Euro Surveill. 2015;20:30060. DOIPubMedGoogle Scholar
  13. Centers for Disease Control and Prevention. Human influenza virus real-time RT-PCR detection and characterization panel. 510(k) summary. 2008. http://www.accessdata.fda.gov/cdrh_docs/pdf8/k080570.pdf.
  14. Zhou  B, Donnelly  ME, Scholes  DT, St George  K, Hatta  M, Kawaoka  Y, et al. Single-reaction genomic amplification accelerates sequencing and vaccine production for classical and Swine origin human influenza a viruses. J Virol. 2009;83:10309–13. DOIPubMedGoogle Scholar
  15. Ma  LC, Guan  R, Hamilton  K, Aramini  JM, Mao  L, Wang  S, et al. A second RNA-binding site in the NS1 protein of influenza B virus. Structure. 2016;24:1562–72. DOIPubMedGoogle Scholar

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