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Volume 23, Number 5—May 2017
Dispatch

Virulence Analysis of Bacillus cereus Isolated after Death of Preterm Neonates, Nice, France, 2013

Romain Lotte, Anne-Laure Hérissé, Yasmina Berrouane, Laurène Lotte, Florence Casagrande, Luce Landraud, Sabine Herbin, Nalini Ramarao, Laurent BoyerComments to Author , and Raymond RuimyComments to Author 
Author affiliations: Centre Hospitalier Universitaire de Nice, Nice, France (R. Lotte, A.-L. Hérissé, Y. Berrouane, L. Lotte, F. Casagrande, L. Landraud, R. Ruimy); Université de Nice Côte d’Azur, INSERM, C3M, Nice (R. Lotte, L. Lotte, L. Landraud, L. Boyer, R. Ruimy); INSERM U1065, C3M, Team 6, Nice (R. Lotte, L. Boyer, R. Ruimy); Food Safety Laboratory, Maison Alfort, France (S. Herbin); Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France (N. Ramarao)

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Figure 2

Genetic and virulence analyses of Bacillus spp. strains isolated from 2 preterm neonates with B. cereus infection and environmental sampling from intensive care unit, Nice, France, 2013. A) Molecular typing using M13-PCR methods, as described by Guinebretiere et al. (5). Lane 1, DNA ladder; lane 2, tracheobronchial fluid, first newborn; lane 3, blood culture, second newborn; lane 4, catheter, second newborn; lane 5, incubator surface, first newborn (Env1); lane 6, incubator surface, first newbor

Figure 2. Genetic and virulence analyses of Bacillus spp. strains isolated from 2 preterm neonates with B. cereus infection and environmental sampling from intensive care unit, Nice, France, 2013. A) Molecular typing using M13-PCR methods, as described by Guinebretiere et al. (5). Lane 1, DNA ladder; lane 2, tracheobronchial fluid, first newborn; lane 3, blood culture, second newborn; lane 4, catheter, second newborn; lane 5, incubator surface, first newborn (Env1); lane 6, incubator surface, first newborn (Env2); lane 7, incubator surface, first newborn (Env3); lane 8, ultrasonographic probe (Env4); lane 9, bench surface used for bottle feeding (Env5); lane 10, incubator surface, second newborn (1); lane 11, incubator surface, second newborn (2); lane 12, negative control; lane 13, DNA ladder. B) Survival of flies infected with Escherichia coli CIP 102181, Staphylococcus aureus CIP 110856, and B. cereus CIP 66.24 T, compared with survival of control flies injected with phosphate-buffered saline (PBS). *p <0.01; **p <0.001; ns, not significant (all by Gehan-Breslow-Wilcoxon χ2 test). C) Survival of flies infected with the different strains of B. cereus tested. Env, environmental; ns, not significant by Gehan-Breslow-Wilcoxon χ2 test.

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