Volume 23, Number 9—September 2017
Dispatch
Norovirus in Bottled Water Associated with Gastroenteritis Outbreak, Spain, 2016
Table
Human norovirus genome copies per liter in analyzed water cooler samples concentrated by glass wool filtration and polyethylene glycol precipitation, metropolitan area of Barcelona, Spain, April 15–20, 2016*
Sample | RT-qPCR |
PMA/T |
|||
---|---|---|---|---|---|
GI | GII | GI | GII | ||
1 | 1.1 × 103 | 5.8 × 103 | 49 | 327 | |
2 | 1.0 × 104 | 2.6 × 104 | 33 | 660 | |
3 | ND | ND | NT | NT | |
4 | ND | ND | NT | NT |
*G, genogroup; ND, not detected; NT, not tested; PMA/T, treatment with the nucleic acid intercalating dye propidium monoazide (PMA, 50 µmol/L) and Triton X surfactant (0.5%) before RT-qPCR; RT-qPCR, real-time TaqMan reverse transcription PCR.
1These authors contributed equally to this article.
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