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Volume 23, Number 9—September 2017

Dispatch

Norovirus in Bottled Water Associated with Gastroenteritis Outbreak, Spain, 2016

Albert Blanco1, Susana Guix1, Noemí Fuster, Cristina Fuentes, Rosa Bartolomé, Thais Cornejo, Rosa Maria Pintó, and Albert BoschComments to Author 
Author affiliations: University of Barcelona, Barcelona, Spain (A. Blanco, S. Guix, N. Fuster, C. Fuentes, R.M. Pintó, A. Bosch); Hospital Universitari Vall d'Hebron, Barcelona (R. Bartolomé, T. Cornejo)

Main Article

Table

Human norovirus genome copies per liter in analyzed water cooler samples concentrated by glass wool filtration and polyethylene glycol precipitation, metropolitan area of Barcelona, Spain, April 15–20, 2016*

Sample RT-qPCR
PMA/T
GI GII GI GII
1 1.1 × 103 5.8 × 103 49 327
2 1.0 × 104 2.6 × 104 33 660
3 ND ND NT NT
4 ND ND NT NT

*G, genogroup; ND, not detected; NT, not tested; PMA/T, treatment with the nucleic acid intercalating dye propidium monoazide (PMA, 50 µmol/L) and Triton X surfactant (0.5%) before RT-qPCR; RT-qPCR, real-time TaqMan reverse transcription PCR.

Main Article

1These authors contributed equally to this article.

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