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Volume 23, Number 9—September 2017

Dispatch

Evaluation of 5 Commercially Available Zika Virus Immunoassays

David SafronetzComments to Author , Angela Sloan, Derek R. Stein, Emelissa Mendoza, Nicole Barairo, Charlene Ranadheera, Leanne Scharikow, Kimberly Holloway, Alyssia Robinson, Maya Traykova-Andonova, Kai Makowski, Kristina Dimitrova, Elizabeth Giles, Joanne Hiebert, Rhonda Mogk, Sharla Beddome, and Michael Drebot
Author affiliations: Public Health Agency of Canada, Winnipeg, Manitoba, Canada

Main Article

Table 1

Results of in-house and commercially available Zika virus immunoassays*

Sample collection dpo In-house Zika virus diagnostic results
DENV PRNT titer Commercial Zika virus serologic assays results
RT-PCR MAC-ELISA PRNT titer Euroimmun IgM Euroimmun IgG Novatec IgM Abcam IgM InBios IgM
12 ND Pos >40 Neg Pos Pos Pos Pos Pos
9 ND Pos >40 Neg Neg Neg Neg Neg Pos
4 ND Pos >40 Neg Neg Neg Neg Pos Pos
27 ND Pos >40 Neg Pos Pos Pos Pos Pos
39 ND Pos >40 Neg Neg Pos Pos Pos Pos
11 ND Pos >40 Neg Pos Pos Pos Pos Pos
109 ND Pos 1,280 20 Neg Pos Neg Neg Pos
49 ND Pos >40 Neg Neg Pos Pos Pos Pos
Unknown ND Pos >40 Neg Neg Neg Neg Neg Pos
4 ND Pos >40 Neg Pos Neg Pos Pos Pos
7 ND Pos >40 Neg Neg Pos Neg Neg Pos
46 ND Pos >40 Neg Neg Pos Pos Pos Pos
Unknown ND Pos >40 Neg Neg Pos Neg Neg Pos
Unknown ND Pos >40 Neg Neg Pos Eq Pos Pos
118 ND Pos >40 Neg Neg Pos Neg Neg Pos
57 ND Pos >40 Neg Pos Neg Pos Pos Pos
66 ND Pos >40 Neg Neg Pos Neg Eq Pos
43 ND Pos 40 Neg Neg Pos Eq Pos Pos
2 ND Pos >40 Neg Neg Neg Pos Neg Pos
41 ND Pos >40 Neg Pos Pos Neg Neg Pos
5 ND Pos >40 Neg Neg Neg Neg Neg Pos
38 ND Pos >40 Neg Neg Pos Neg Neg PZ
4 ND Pos >80 Neg Pos Neg Neg Pos Pos
6 ND Pos >80 Neg Pos Pos Neg Neg Pos
2 ND Pos >80 Neg Neg Pos Neg Eq Pos
12 ND Pos 40 Neg Pos Neg Pos Pos Pos
Unknown ND Pos >80 Neg Pos Neg Pos Pos Pos
28 ND Pos >80 Neg Pos Pos Neg Neg Pos
75 ND Pos >80 20 Neg Pos Neg Neg Pos
68 ND Pos >40 Neg Neg Pos Neg Neg Pos
9 ND Pos Neg >40 Neg Neg Neg Neg PZ
7 ND Pos Neg >40 Neg Neg Neg Neg PZ
31 ND Pos Neg >40 Neg Neg Neg Neg PZ
6 ND Pos Neg >40 Pos Pos Neg Neg Pos
20 ND Pos Neg >40 Neg Neg Pos Pos PZ
36 ND Pos Neg >80 Neg Neg Neg Neg OF
Unknown ND Pos 320 >5,120 Neg Neg Neg Neg Pos
Unknown ND Pos Neg 40 Neg Neg Pos Neg Neg
3 ND Pos Neg >80 Neg Neg Eq Neg Pos
Unknown ND Pos Neg >640 Neg Neg Neg Neg Pos

*Bold indicates false-positive/false-negative results. Underlining indicates inconclusive results that required further testing. DENV, dengue virus; dpo, days postsymptom onset; Eq, equivalent; MAC-ELISA, IgM-capture ELISA; ND, not done; Neg, negative; OF, other flavivirus; Pos, positive; PRNT, plaque reduction neutralization test; PZ, possible Zika virus; RT-PCR, reverse transcription PCR.

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