Volume 23, Number 9—September 2017
Research
Molecular Antimicrobial Resistance Surveillance for Neisseria gonorrhoeae, Northern Territory, Australia
Table 1
PCR results for the 1,629 NAAT-positive Neisseria gonorrhoeae clinical samples taken from patients in the Northern Territory of Australia, 2014*
Assay and targets |
Results | |||||||
---|---|---|---|---|---|---|---|---|
CAZ region, n = 441 |
ZAP region, n = 1,188 |
Total for both regions, n = 1,629 |
||||||
No. samples/no. tested |
% Samples (95% CI) |
No. samples/no. tested |
% Samples (95% CI) |
No. samples/no. tested |
% Samples (95% CI) |
|||
GyrA91-PCR (gyrA) | ||||||||
Wild type (S91) | 235/297 | 79.1 (74.1–83.4) | 922/931 | 99.0 (98.2–99.5) | 1,157/1,228 | 94.2 (92.8–95.4) | ||
S91F | 62/297 | 20.9 (16.6–25.9) | 9/931 | 1.0 (0.5–1.8) | 71/1,228 | 5.8 (4.6–7.2) | ||
Uncharacterized† |
144 |
32.6 |
257 |
21.6 |
401/1,629 |
24.6 |
||
PPNG-PCR | ||||||||
Negative | 252/286 | 88.1 (83.8–91.4) | 879/888 | 99 (98.1–99.5) | 1,131/1,174 | 96.3 (95.1–97.3) | ||
PPNG | 34/286 | 11.9 (8.6–16.2) | 9/888 | 1 (0.5–1.9) | 43/1,174 | 3.7 (2.7–4.9) | ||
Uncharacterized† |
155 |
35.1 |
300 |
25.3 |
455/1,629 |
27.9 |
||
2611-PCR (23S rRNA) | ||||||||
Wild type | 321/321 | 100 (98.8–100) | 958/960 | 99.8 (99.2–99.9) | 1,279/1,281 | 99.8 (99.4–100) | ||
C2611T | 0/321 | 0 (0–1.2) | 2/960 | 0.2 (0.1–0.8) | 2/1,281 | 0.2 (0–0.6) | ||
Uncharacterized† |
120 |
27.2 |
228 |
19.2 |
348/1,629 |
21.4 |
||
2059-PCR (23S rRNA) | ||||||||
Wild type | 319/319 | 100 (98.8–100) | 1,004/1,004 | 100 (99.6–100) | 1,323/1,323 | 100 (99.7–100) | ||
A2059G | 0/319 | 0 (0–1.2) | 0/1,004 | 0 (0–0.4) | 0/1,323 | 0 (0–0.3) | ||
Uncharacterized† |
122 |
27.7 |
184 |
15.5 |
306/1,629 |
18.8 |
||
PorB-PCR (porB) | ||||||||
G101/A102 | 98/286 | 34.3 (29–39.9) | 125/835 | 15.0 (12.7–17.6) | 223/1,121 | 19.9 (17.7–22.3) | ||
[G101/A102, non-PPNG]‡ | [80/280] | [28.6 (23.4–34.1)] | [116/823] | [14.1 (11.9–16.6)] | [196/1,103] | [17.8 (15.6–20.1)] | ||
PorB1a | 152/286 | 53.1 (47.4–58.9) | 699/835 | 83.7 (81.1–86.1) | 851/1,121 | 75.9 (73.3–78.3) | ||
[PorB1a, non-PPNG]‡ | [120/267] | [44.9 (39.1–50.9)] | [627/770] | [81.3 (78.4–83.9)] | [747/1,037] | [72.0 (69.2–74.7)] | ||
Mutant§ | 32/286 | 11.2 (8–15.4) | 3/835 | 0.4 (0.1–1.1) | 35/1,121 | 3.1 (2.3–4.3) | ||
PorB1a and G101/A102 | 4/286 | 1.4 (0.5–3.5) | 6/835 | 0.7 (0.3–1.6) | 10/1,121 | 0.9 (0.5–1.6) | ||
PorB1a and mutant§ | 0/286 | 0 (0–1.3) | 2/835 | 0.2 (0.1–0.9) | 2/1,121 | 0.2 (0–0.6) | ||
Uncharacterized† |
155 |
35.1 |
353 |
29.8 |
508/1,629 |
31.2 |
||
A-deletion-PCR (MtrR) | ||||||||
Wild type | 280/318 | 88.1 (84–91.2) | 944/950 | 99.4 (98.6–99.7) | 1,224/1,268 | 96.5 (95.4–97.4) | ||
[Wild type, non-PPNG]‡ | [226/284] | [79.6 (74.5–84.1)] | [861/873] | [98.6 (97.6–99.2)] | [1,087/1,157] | [93.9 (92.4–95.2)] | ||
A-deletion | 38/318 | 11.9 (8.8–16) | 6/950 | 0.6 (0.3–1.4) | 44/1268 | 3.5 (2.6–4.6) | ||
Uncharacterized† |
123 |
27.9 |
238 |
20 |
361/1629 |
22.2 |
||
mosaicPBP2-PCR (penA) | ||||||||
Negative | 431/441 | 97.7 (95.9–98.8) | 1,188/1,188 | 100 (99.7–100) | 1,619/1,629 | 99.4 (98.9–99.7) | ||
Positive | 10/441 | 2.3 (1.2–4.1) | 0/1188 | 0 (0–0.3) | 10/1,629 | 0.6 (0.3–1.1) | ||
Uncharacterized†¶ |
NA |
NA |
NA |
NA |
NA |
NA |
||
A8806-PCR (penA) | ||||||||
Negative | 441/441 | 100 (99.1–100) | 1,188/1,188 | 100 (99.7–100) | 1,629/1,629 | 100 (99.8–100) | ||
Positive | 0/441 | 0 (0–0.9) | 0/1,188 | 0 (0–0.3) | 0/1,629 | 0 (0–0.2) | ||
Uncharacterized†¶ | NA | NA | NA | NA | NA | NA |
*NA, not applicable; NAAT, nucleic acid amplification test; PPNG, penicillinase-producing N. gonorrhoeae.
†These samples could not be characterized by the respective PCR assay.
‡These PorB-PCR and A-deletion-PCR results were combined with the PPNG-PCR results to exclude those that were PPNG. Uncharacterized PPNG-PCR results were omitted from total numbers.
§Mutant = G101K/A102D, G101K/A102N or G101K/A102G.
¶All negative results for the mosaicPBP2-PCR and A8806-PCR were considered valid (i.e., characterized) given that pooled samples were used for screening, and it was therefore not possible to ascertain characterization for individual samples.