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Volume 23, Number 9—September 2017

Genetic Diversity of Highly Pathogenic Avian Influenza A(H5N8/H5N5) Viruses in Italy, 2016–17

Alice Fusaro1Comments to Author , Isabella Monne1, Paolo Mulatti, Bianca Zecchin, Lebana Bonfanti, Silvia Ormelli, Adelaide Milani, Krizia Cecchettin, Philippe Lemey, Ana Moreno, Paola Massi, Tiziano Dorotea, Stefano Marangon, and Calogero Terregino
Author affiliations: Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy (A. Fusaro, I. Monne, P. Mulatti, B. Zecchin, L. Bonfanti, S. Ormelli, A. Milani, K. Cecchettin, T. Dorotea, S. Marangon, C. Terregino); Rega Institute, KU Leuven, Leuven, Belgium (P. Lemey); Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Brescia, Italy (A. Moreno, P. Massi).

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In winter 2016–17, highly pathogenic avian influenza A(H5N8) and A(H5N5) viruses of clade were identified in wild and domestic birds in Italy. We report the occurrence of multiple introductions and describe the identification in Europe of 2 novel genotypes, generated through multiple reassortment events.

In spring 2016, highly pathogenic avian influenza (HPAI) outbreaks caused by the H5N8 subtype of clade (group B) were reported in migratory wild birds in Qinghai Lake, China (1), and in the salt lake system of Uvs Nuur on the Russian Federation–Mongolia border (2). Since then, HPAI A(H5N8) viruses have been detected in several countries in Asia, Europe, and Africa. In Europe, the virus was detected for the first time in October 2016 in Hungary (3). Here, we describe the occurrence of multiple introductions of reassortant HPAI A(H5N8) and A(H5N5) viruses in Italy, in both wild and domestic birds.

The Study

Figure 1

Thumbnail of Highly pathogenic avian influenza A(H5N8) and A(H5N5) in birds, Italy, 2016–17). A) Geographic distribution of cases in wild (red) and domestic (blue) birds in northern Italy. Squares indicate the samples sequenced in this study; circles indicate positive samples for which no genetic information was available at the time of writing. B) Maximum likelihood phylogenetic tree of the hemagglutinin gene of clade viruses. Viruses analyzed in this study are indicated with red (wild

Figure 1. Highly pathogenic avian influenza A(H5N8) and A(H5N5) in birds, Italy, 2016–17). A) Geographic distribution of cases in wild (red) and domestic (blue) birds in northern Italy. Squares indicate the samples sequenced...

During December 2016–January 2017, a Eurasian wigeon (Anas penelope) and a gadwall (Anas strepera) found dead at Grado Lagoon in northeastern Italy tested positive for HPAI A(H5N5). A second wigeon tested positive for HPAI A(H5N8). Since then, additional HPAI A(H5N8) cases were observed in a common shelduck (Tadorna tadorna) and in a mute swan (Cygnus olor) and in birds on 6 commercial turkey farms, 1 layer farm, and 3 backyard flocks (Table 1; Figure 1). All of the cases in domestic poultry farms occurred in areas in close proximity to wetlands that are listed as important resting sites for migratory waterfowl. The onset of clinical signs in all the affected poultry species was generally associated with depression, reluctance to move, and a drop in feed consumption. The clinical condition often evolved into a more severe respiratory and nervous syndrome associated with an increased mortality rate (average mortality rate is 1.62% [95% CI 1.10%–2.14%]). Depopulation measures on the infected farms and 7 neighboring poultry premises considered at risk involved ≈510,000 birds.

The genomes of 10 positive samples collected from wild (n = 4) and domestic (n = 6) birds were fully sequenced (Technical Appendix 1). Phylogenetic analysis of the hemagglutinin (HA) gene showed that the HPAI A(H5N5) and A(H5N8) viruses clustered within the clade, group B (Figure 1). However, the characterization of the complete genome (Technical Appendix 1 Figures 1–8) revealed that these viruses belong to 4 distinct genotypes, which had very likely originated from multiple reassortment events.

Figure 2

Thumbnail of Probable genesis of highly pathogenic avian influenza A(H5N8) and A(H5N5) reassortant viruses identified in Italy, 2016–17 (gray box). Virus particles are represented by ovals containing horizontal bars that represent the 8 gene segments, colored according to their origin.

Figure 2. Probable genesis of highly pathogenic avian influenza A(H5N8) and A(H5N5) reassortant viruses identified in Italy, 2016–17 (gray box). Virus particles are represented by ovals containing horizontal bars that represent the 8...

Phylogenetic analyses indicated that the HPAI H5N5 viruses had been generated through intersubtype reassortment events between the H5N8 viruses from Asia (H5N8-Gs/Qinghai/2016-like) and the low pathogenicity avian influenza (LPAI) viruses of the Eurasian lineage (Figure 2). The A(H5N8) viruses from Asia were the source of the HA, polymerase acidic, matrix, and nonstructural protein genes. HPAI A(H5N5) viruses with similar HA and neuraminidase genes were identified in Croatia and Czech Republic in 2016–17. The time to the most recent common ancestor (tMRCA) estimated by pooling the information across all the gene segments in a hierarchical model (Technical Appendix 1) suggested that a virus with this gene constellation emerged during October–December 2016 (Table 2; Technical Appendix 1 Table 1).

Among the 8 HPAI A(H5N8) viruses in Italy investigated during this study, 5 were collected from wild and domestic birds in the Veneto region. In all the phylogenetic trees, these viruses clustered within the main European A(H5N8) group (A/wild duck/Poland/82A/2016-like) (Figure 2), previously described by Pohlmann et al. (4). The tMRCA for this group was May–June 2016 in the hierarchical gene segment model (Table 2; Technical Appendix 1 Table 1). The first HPAI A(H5N8) virus detected in a turkey farm in the Veneto region displayed the gene composition of a virus isolated in October 2016 from a painted stork in an Indian zoo (5), which had not previously been reported in Europe (Figure 2). The tMRCA of this Indian–Italian group is July–October 2016, according to the hierarchical gene segment model (Table 2; Technical Appendix 1 Table 1). The 2 outbreaks reported in 2 commercial turkey farms in the Emilia-Romagna and Lombardy regions were caused by HPAI A(H5N8) reassortant viruses containing the polymerase basic protein 2 and nucleoprotein genes of LPAI viruses of the Eurasian lineage and the remaining genes from the H5N8-Gs/Qinghai/2016-like genotype (Figure 2). Viruses with a similar gene pool were identified in Croatia and France. Estimation of the tMRCA by the hierarchical gene segment model indicated that this genotype might have emerged during June–August 2016 (Table 2; Technical Appendix 1 Table 1).

Analyses of the phylogenetic topologies revealed that most of the sequences found in Italy were dispersed throughout the trees, indicating the occurrence of several independent introductions of the A(H5N8) virus into poultry farms from wild birds (Technical Appendix 1 Figures 1–8). These results were confirmed by our median-joining network analyses for the HA gene (Technical Appendix 1 Figure 9), which showed that the ancestral sequences of the samples from Italy represent viruses collected in other countries. In most cases >1 median vector, representing the lost ancestral sequences, separated these viruses from the hypothetical progenitor. The only exception was for A/turkey/Italy/17VIR576-11/2017 and A/turkey/Italy/17VIR1452-22/2017, which proved to be almost identical for all the genes (similarity of 99.9%–100%), although they were collected 24 days apart in 2 turkey flocks located at a distance of ≈90 km from one another and no evident contacts were observed between them. However, because the 2 outbreaks had occurred in 2 farms operated by the same company, an exchange of virus cannot be ruled out.

Intravenous pathogenicity indexes obtained for 8 representative A(H5N8) and A(H5N5) isolates ranged from 2.85–3, comparable to an index of 2.93 for 2016 A(H5N8) viruses from Germany and 2.75–2.84 for 2016 A(H5N8) viruses from Russia (2,4). These data confirm that both of the A(H5N8) and A(H5N5) viruses from Italy, which shared the same HA cleavage site (PLREKRRKR), are highly pathogenic for poultry.


Since its emergence in China in 2013, the HPAI H5 of clade has evolved in different genetic groups, namely A to D (6). Here, we describe the introductions of 4 different H5 viral genotypes of clade group B in northern Italy. As previously observed for the 2014–15 A(H5N8) epidemic wave (7), our results confirm that these strains have a high propensity to reassort with co-circulating LPAI and HPAI viruses, causing the generation of several subtypes and genotypes with unique gene constellations. Unfortunately, the lack of sequences of the potential progenitors, exemplified by the long branches observed in particular in the polymerase basic protein 2, polymerase acidic, and nucleoprotein phylogenies, makes it difficult to determine when and where these genotypes emerged. The genetic variability observed in the viruses identified in domestic birds, the similarity to viruses circulating in Europe and India, and the close proximity of the infected poultry farms to wetlands all suggest that wild birds did play a major role in the multiple and independent introductions of the virus into poultry holdings.

Our study highlights the importance of generating complete viral genome sequences in a timely fashion, which may help to monitor the viral spread and define appropriate disease control strategies. This, coupled with intensified wild bird surveillance on wetlands of ecologic importance for avian influenza viruses, can improve our understanding of the virus dissemination routes and support early detection of viruses highly pathogenic to poultry or believed to be of immediate concern to human health.

Dr. Fusaro is a biotechnologist working at the Istituto Zooprofilattico Sperimentale delle Venezie. Her primary research interests include molecular epidemiology, intra- and inter-host evolution, gene flow, and cross-species transmission of RNA viruses.



We acknowledge the authors and the originating and submitting laboratories of the sequences from the GISAID EpiFlu Database on which this research is based in part (Technical Appendix 2). We also thank Annalisa Salviato, Alessia Schivo, Sabrina Marciano, and Francesca Ellero for their excellent technical assistance.

This study was financially supported by the European project PREDEMICS (research project supported by the European Community’s Seventh Framework Programme [FP7/2007-2013] under grant agreement 278433) and the Italian Ministry of Health (RC IZSVe 05/14).



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Cite This Article

DOI: 10.3201/eid2309.170539

1These authors contributed equally to this article.

Table of Contents – Volume 23, Number 9—September 2017

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Please use the form below to submit correspondence to the authors or contact them at the following address:

Alice Fusaro, Istituto Zooprofilattico Sperimentale delle Venezie, Research & Innovation Department, OIE and National Reference Laboratory for avian influenza & Newcastle disease, FAO Reference Centre for animal influenza and Newcastle disease, OIE Collaborating Centre for Diseases at the Human-Animal Interface, Viale dell’Università 10, 35020, Legnaro, Padua, Italy

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Page created: August 17, 2017
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