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Volume 24, Number 12—December 2018
Research

Genomic Characterization of β-Glucuronidase–Positive Escherichia coli O157:H7 Producing Stx2a

Yoshitoshi OguraComments to Author , Kazuko Seto, Yo Morimoto, Keiji Nakamura, Mitsuhiko P. Sato, Yasuhiro Gotoh, Takehiko Itoh, Atsushi Toyoda, Makoto Ohnishi, and Tetsuya Hayashi
Author affiliations: Kyushu University, Fukuoka, Japan (Y. Ogura, K. Nakamura, M.P. Sato, Y. Gotoh, T. Hayashi); Osaka Institute of Public Health, Osaka, Japan (K. Seto); Hokkaido Institute of Public Health, Hokkaido, Japan (Y. Morimoto); Tokyo Institute of Technology, Tokyo, Japan (T. Itoh); National Institute of Genetics, Shizuoka, Japan (A. Toyoda); National Institute of Infectious Diseases, Tokyo (M. Ohnishi)

Main Article

Figure 2

Whole-genome sequence-based phylogenetic analysis and repertoires of T3SS effectors and plasmid-encoding virulence factors from the study of Stx–producing E. coli O157:H7. The genome sequences of all the strains used in this study were aligned with the complete chromosome sequence of Sakai, and the single-nucleotide polymorphisms located in the 4,074,209-bp backbone sequence that were conserved in all the test strains were identified. After removing the recombinogenic single-nucleotide polymorph

Figure 2. Whole-genome sequence-based phylogenetic analysis and repertoires of T3SS effectors and plasmid-encoding virulence factors from the study of Stx–producing E. coli O157:H7. The genome sequences of all the strains used in this study were aligned with the complete chromosome sequence of Sakai, and the single-nucleotide polymorphisms located in the 4,074,209-bp backbone sequence that were conserved in all the test strains were identified. After removing the recombinogenic single-nucleotide polymorphisms sites, we performed a concatenated alignment of 5,803 informative sites to generate the maximum-likelihood phylogeny. The conservation of T3SS effectors and plasmid-encoding virulence factors is shown in the tree. Colored boxes indicate the presence, and open boxes the absence, of each gene. GP, β-glucuronidase–positive; LEE, locus of enterocyte effacement; SF, sorbitol-fermenting. Scale bars indicate nucleotide substitutions per site.

Main Article

Page created: November 20, 2018
Page updated: November 20, 2018
Page reviewed: November 20, 2018
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