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Volume 24, Number 3—March 2018

Emergence of Streptococcus pneumoniae Serotype 12F after Sequential Introduction of 7- and 13-Valent Vaccines, Israel

Assaf RokneyComments to Author , Shalom Ben-Shimol, Zinaida Korenman, Nurith Porat, Zeev Gorodnitzky, Noga Givon-Lavi, Merav Ron, Vered Agmon, Ron Dagan, and Lea Valinsky
Author affiliations: Ministry of Health, Jerusalem, Israel (A. Rokney, Z. Korenman, Z. Gorodnitzky, M. Ron, V. Agmon, L. Valinsky); Soroka University Medical Center, Beer Sheva, Israel (S. Ben-Shimol, N. Porat, N. Givon-Lavi); Ben-Gurion University of the Negev, Beer Sheva (S. Ben-Shimol, N. Porat, N. Givon-Lavi, R. Dagan)

Main Article

Table 2

Multilocus sequence typing PCR amplification and sequencing primers for study of Streptococcus pneumoniae serotypes, Israel, July 2009–June 2016

Primers Sequence, 5′ → 3′
aroE/M13F TGTAAAACGACGGCCAGTcgtttagctgcagttgttgc
aroE/M13R CAGGAAACAGCTATGACCcccacactggtggcattaac
ddl/M13F TGTAAAACGACGGCCAGTttgccatggataaaacacgac
ddl/M13R CAGGAAACAGCTATGACCcgcgcttgtcaaaactttcc
gdh/M13F TGTAAAACGACGGCCAGTgtgctgaaaagattaaggtct
gdh/M13R CAGGAAACAGCTATGACCtgcttccagctttatagtcatg
gki/M13F TGTAAAACGACGGCCAGTggcattggaatgggatcacc
gki/M13R CAGGAAACAGCTATGACCtctcccgcagctgacac
recP/M13F TGTAAAACGACGGCCAGTgccaactcaggtcatccagg
recP/M13R CAGGAAACAGCTATGACCttcgatagcagcatggatgg
spi/M13F TGTAAAACGACGGCCAGTcgcttagaaaggtaagttatg
spi/M13R CAGGAAACAGCTATGACCaggctgagattggtgattctc
xpt/M13F TGTAAAACGACGGCCAGTggaggtcttatgaaattattag

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Page updated: February 15, 2018
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