Genetic Characterization of Enterovirus A71 Circulating in Africa
Maria Dolores Fernandez-Garcia
1 , Romain Volle
1, Marie-Line Joffret, Serge Alain Sadeuh-Mba, Ionela Gouandjika-Vasilache, Ousmane Kebe, Michael R. Wiley, Manasi Majumdar, Etienne Simon-Loriere, Anavaj Sakuntabhai, Gustavo Palacios, Javier Martin, Francis Delpeyroux, Kader Ndiaye
2, and Maël Bessaud
2
Author affiliations: Institut Pasteur, Dakar, Senegal (M.D. Fernandez-Garcia, O. Kebe, K. Ndiaye); Institut Pasteur, Paris, France (R. Volle, M.-L. Joffret, E. Simon-Loriere, A. Sakuntabhai, F. Delpeyroux, M. Bessaud); Institut National de la Santé et de la Recherche Médicale, U994, Paris (R. Volle, M.-L. Joffret, F. Delpeyroux); Centre Pasteur, Yaoundé, Cameroon (S.A. Sadeuh-Mba); Institut Pasteur, Bangui, Central African Republic (I. Gouandjika-Vasilache); University of Nebraska Medical Center, Omaha, Nebraska, USA (M.R. Wiley); US Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA (M.R. Wiley, G. Palacios); National Institute for Biological Standards and Control, Hertfordshire, UK (M. Majumdar, J. Martin)
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Figure 2
Figure 2. Identification of recombinant sequences in the genome of EV-A71 C2 isolates from patients with acute flaccid paralysis in Africa (14-157, 14-250, 13-365, 15-355) by similarity plot against potential parent genomes (CV-A14 strain 14-254; EV-A71 genogroup E strains 13-194, 08-146, and 03-008) and from GenBank (CV-A10, CV-A5, EV-A120). Similarity plot analysis was performed using SimPlot version 3.5.1 (http://sray.med.som.jhmi.edu/SCRoftware/simplot) on the basis of full-length genomes. For the analysis, we used a window of 600 nt moving in 20-nt steps. Approximate nt positions in the enterovirus genome are indicated. The enterovirus genetic map is shown in the top panel. We used the genome of EV-A71 study strain 14-157 as a query sequence. UTR, untranslated region.
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