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Volume 24, Number 5—May 2018
Research Letter

Rickettsia asembonensis Characterization by Multilocus Sequence Typing of Complete Genes, Peru

Steev Loyola, Carmen Flores-Mendoza, Armando Torre, Claudine Kocher, Melanie Melendrez, Alison Luce-Fedrow, Alice N. Maina, Allen L. Richards, and Mariana LeguiaComments to Author 
Author affiliations: US Naval Medical Research Unit No. 6, Lima, Peru (S. Loyola, C. Flores-Mendoza, A. Torre, C. Kocher, M. Leguia); Pontificia Universidad Católica del Perú, Lima (A. Torre, M. Leguia); Walter Reed Army Institute of Research, Silver Spring, Maryland, USA (M. Melendrez); Naval Medical Research Center, Silver Spring (A. Luce-Fedrow, A.N. Maina, A.L. Richards)

Main Article

Table

Multilocus sequence typing analysis of complete genes from Peru Rickettsia asembonensis molecular isolate VGD7*

Changes Conserved genes (GenBank accession no.)
 Variable genes (GenBank accession no.)
17-kDa (KY650696) gltA (KY650697) ompA (KY650698) ompB (KY650699) sca4 (KY650700)
Genome
Complete ORF, nt 480 1,314 5,076 4,947 3,033
Identity, % 100 99.8 99.9 100 99.8
Mutations None C 138 T T 484 G None T 383 C
T 537 C C 828 A T 807 C
A 868 G A 3838 G G 1824 T
C 4360 G A 2260 G
T 4880 C T 2492 C
Total changes
 0
 3
5
 0
 5
Protein
Complete protein, aa 160 438 1,692 1,649 1,011
Identity, % 100 99.8 99.8 100 99.6
Mutations None K 290 E Y 162 D None L 128 P
R 1280 G Q 608 H
L 1454 V R 754 G
V 1627 A I 831 T
Total changes 0 1 4 0 4

*Comparisons were made against reference strain NMRCii from Kenya (GenBank accession no. JWSW01000078.1). Blank cells indicate no additional mutations or changes to report. ORF, open reading frame.

Main Article

Page created: April 17, 2018
Page updated: April 17, 2018
Page reviewed: April 17, 2018
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