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Volume 24, Number 5—May 2018
Research

Dynamics of Spirochetemia and Early PCR Detection of Borrelia miyamotoi

Lyudmila Karan, Marat MakenovComments to Author , Nadezhda Kolyasnikova, Olga Stukolova, Marina Toporkova, and Olga Olenkova
Author affiliations: Central Research Institute of Epidemiology, Moscow, Russia (L. Karan, M. Makenov, N. Kolyasnikova, O. Stukolova); Medical Association Novaya Bolnitsa, Yekaterinburg, Russia (M. Toporkova); Clinical Diagnostic Center, Yekaterinburg (O. Olenkova)

Main Article

Table 1

Cross-validation of detection of Borrelia spp. by quantitative PCR and ELISA in blood samples from 310 patients with suspected borreliosis, Yekaterinburg, Russia, 2010 and 2015*

Quantitative PCR for 16S rRNA gene
 No. patients
 No. ELISA positive
 No. ELISA negative
 ND
With erythema migrans
Borrelia burgdorferi sensu lato 31 30 1 0
B. miyamotoi 5 5 0 0
PCR negative 131 81 39 11
Total
 167
 116
 40
 11
Without erythema migrans
B. burgdorferi sensu lato 4 4 0 0
B. miyamotoi 65 55 10 0
B. burgdorferi sensu lato plus B. miyamotoi 1 0 1 0
PCR negative 73 73 0 0
Total 143 132 11 0

*ELISA results are for borrelial IgM or IgG seroconversion. ND, not determined.

Main Article

Page created: April 17, 2018
Page updated: April 17, 2018
Page reviewed: April 17, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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