Human Usutu Virus Infection with Atypical Neurologic Presentation, Montpellier, France, 2016
Yannick Simonin
, Olivier Sillam, Marie J. Carles, Serafin Gutierrez, Patricia Gil, Orianne Constant, Marie F. Martin, Gilda Grard, Philippe Van de Perre, Sara Salinas, Isabelle Leparc-Goffart, and Vincent Foulongne
Author affiliations: Université de Montpellier, Montpellier, France (Y. Simonin, O. Constant, M.F. Martin, P. Van de Perre, S. Salinas, V. Foulongne); Université de Montpellier Hôpital, Montpellier (O. Sillam); Nimes University Hospital, Nimes, France (M.J. Carles); Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Montpellier (S. Gutierrez, P. Gil); Institut de Recherche Biomédicale des Armées, Marseille, France (G. Grard, I. Leparc-Goffart)
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Figure 1
Figure 1. Phylogenetic relationship of the France-Montpellier-2016 strain of Usutu virus (USUV) (box; GenBank accession no. LT854220), isolated from a 39-year-old man in Montpellier, France, who had an atypical neurologic presentation, compared with other USUV strains based on the partial nonstructural protein 5 gene sequence. USUV sequences are shown with their country of isolation, year of isolation, and GenBank accession numbers. Hosts from which the strains were detected (bird, mosquito, bat, or human) are shown next to strain names. Analysis was processed through the French phylogeny website (http://www.phylogeny.fr). Nucleotide sequences were aligned by using MUSCLE software (https://www.ebi.ac.uk/Tools/msa/muscle/). The phylogenetic tree was constructed by using the maximum-likelihood method in PhyML (http://www.atgc-montpellier.fr/phyml/). One hundred bootstrap datasets with random sequence addition were computed to generate a consensus tree drawn with TreeDyn software (http://www.treedyn.org/) and rooted with a West Nile virus sequence (GenBank accession no. AF196835). Numbers along branches are bootstrap values. Map shows locations where USUV strains were detected in France during 2015–2016. Scale bar indicates nucleotide substitutions per site.
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