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Volume 24, Number 5—May 2018
Dispatch

Human Usutu Virus Infection with Atypical Neurologic Presentation, Montpellier, France, 2016

Yannick SimoninComments to Author , Olivier Sillam, Marie J. Carles, Serafin Gutierrez, Patricia Gil, Orianne Constant, Marie F. Martin, Gilda Grard, Philippe Van de Perre, Sara Salinas, Isabelle Leparc-Goffart, and Vincent Foulongne
Author affiliations: Université de Montpellier, Montpellier, France (Y. Simonin, O. Constant, M.F. Martin, P. Van de Perre, S. Salinas, V. Foulongne); Université de Montpellier Hôpital, Montpellier (O. Sillam); Nimes University Hospital, Nimes, France (M.J. Carles); Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Montpellier (S. Gutierrez, P. Gil); Institut de Recherche Biomédicale des Armées, Marseille, France (G. Grard, I. Leparc-Goffart)

Main Article

Figure 2

Cerebrospinal fluid sample of a 39-year-old man in Montpellier, France, infected with USUV who had an atypical neurologic presentation. The sample was amplified for 6 days on C636 cells, and the supernatant was used to infect Vero cells or primary human astrocytes. A) Cytopathic effect (presence of adherent dead cells and absence of heaps; all dead cells were scattered) was observed at day 5 postinfection of a Vero cell culture. Scale bar indicates 100 μm. B) Mock or infected primary human astro

Figure 2. Cerebrospinal fluid sample of a 39-year-old man in Montpellier, France, infected with USUV who had an atypical neurologic presentation. The sample was amplified for 6 days on C6/36 cells, and the supernatant was used to infect Vero cells or primary human astrocytes. A) Cytopathic effect (presence of adherent dead cells and absence of heaps; all dead cells were scattered) was observed at day 5 postinfection of a Vero cell culture. Scale bar indicates 100 μm. B) Mock or infected primary human astrocytes were fixed at day 4 postinfection and labeled with pan-flavivirus antibody (MAB10216, clone D1–4G2) by indirect immunofluorescence (green). Strong labeling was observed in some cells (arrows). Nuclei are labeled with DAPI (4′,6-diamidino-2-phenylindole) (blue). NI, not infected; USUV, Usutu virus. Scale bar indicates 10 μm.

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Page created: April 24, 2018
Page updated: April 24, 2018
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