Figure 3. Western blot analysis of dromedary prion protein (PrPSc). A) Western blot analysis of proteinase K (PK)–treated PrPSc in brain homogenates from dromedary camels with neurologic symptoms (#4 and #8), Ouargla abattoir, Algeria. A sample of sheep scrapie was loaded as control (indicated as C+). Membranes were probed with L42 (left) and 12B2 monoclonal antibody (mAb) (right). Molecular weights (kDa) are indicated on the left. Tissue equivalents loaded per lane were 2 mg for camel samples and 0.1 mg for sheep scrapie. B) Samples after deglycosylation. Membrane was probed with L42 mAb. C) comparison of dromedary protease-resistant cores (from camel #4) with sheep BSE, bovine BSE, and sheep scrapie samples by ISS (Istituto Superiore di Sanità) discriminatory Western blot (17). Tissue equivalents loaded per lane were 2 mg for dromedary camel and bovine samples and 0.1 mg for sheep samples. In each blot, samples were loaded as follows: M, molecular weight markers; lane 1, ovine BSE; lane 2, bovine BSE; lane 3, dromedary CPD, camel 4; lane 4, ovine scrapie. Membranes were probed with L42, 12B2 and SAF32 mAbs, as indicated. In B and C, protein standards were loaded and indicated as M. BSE, bovine spongiform encephalopathy; CPD, camel prion disease.