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Volume 24, Number 8—August 2018
Dispatch

Detection of Dengue Virus among Children with Suspected Malaria, Accra, Ghana

Nicholas Amoako, Samuel Duodu, Francis E. Dennis, Joseph H.K. Bonney, Kwaku P. Asante, Juliana Ameh, Lydia Mosi, Takaya Hayashi, Eudosia E. Agbosu, Deborah Pratt, Darwin J. Operario, Barry Fields, Jie Liu, Eric R. Houpt, George E. Armah, Justin Stoler, and Gordon A. AwandareComments to Author 
Author affiliations: University of Ghana, Legon, Ghana (N. Amoako, S. Duodu, F.E. Dennis, J.H.K. Bonney, L. Mosi, T. Hayashi, E.E. Agbosu, D. Pratt, G.E. Armah, G.A. Awandare); Kintampo Health Research Centre, Kintampo, Ghana (N. Amoako, K.P. Asante); Ledzokuku Krowor Municipal Assembly Hospital, Teshie, Accra, Ghana (J. Ameh); Tokyo Medical and Dental University, Tokyo, Japan (T. Hayashi); University of Virginia, Charlottesville, Virginia, USA (D.J. Operario, J. Liu, E.R. Houpt); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (B. Fields); University of Miami, Florida, USA (J. Stoler).

Main Article

Figure 1

TaqMan array card amplification plots for 2 dengue virus–positive samples and 1 negative sample in study of dengue virus among 166 children with suspected malaria, Accra, Ghana, October 2016–July 2017. Blood samples (2.5 mL of the 5.0 mL collected) obtained from children reporting to the hospital with acute febrile illness (AFI) were screened for 26 pathogens simultaneously by using the real-time PCR TaqMan array card. The cards were in 384-well format, and each well contained 1 µL of reaction m

Figure 1. TaqMan array card amplification plots for 2 dengue virus–positive samples and 1 negative sample in study of dengue virus among 166 children with suspected malaria, Accra, Ghana, October 2016–July 2017. Blood samples (2.5 mL of the 5.0 mL collected) obtained from children reporting to the hospital with acute febrile illness (AFI) were screened for 26 pathogens simultaneously by using the real-time PCR TaqMan array card. The cards were in 384-well format, and each well contained 1 µL of reaction mixture (0.75 µL of the extracted total nucleic acid and 0.25 µL of TaqMan Fast Virus 1-step Master Mix; Life Technologies/Applied Biosystems, Foster City, CA, USA). The assays were run on an Applied Biosystems Quant Studio 7 Flex real-time PCR system, according to the manufacturer’s recommendations. Two samples tested positive for dengue virus: A) F227, which amplified with critical Ct of 24.40; and B) F299, which amplified with Ct of 19.35. C) All others tested negative, indicated by amplification signals (ΔRn) below threshold levels at quantification cycle cutoff of 35. Each assay included nucleic acid to serve as amplification controls. External controls were spiked into each to monitor extraction and amplification efficiency, and 1 negative control was included for each batch of extraction to monitor laboratory contamination. Ct, cycle threshold.

Main Article

Page created: July 18, 2018
Page updated: July 18, 2018
Page reviewed: July 18, 2018
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