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Volume 24, Number 8—August 2018

Detection of Dengue Virus among Children with Suspected Malaria, Accra, Ghana

Nicholas Amoako, Samuel Duodu, Francis E. Dennis, Joseph H.K. Bonney, Kwaku P. Asante, Juliana Ameh, Lydia Mosi, Takaya Hayashi, Eudosia E. Agbosu, Deborah Pratt, Darwin J. Operario, Barry Fields, Jie Liu, Eric R. Houpt, George E. Armah, Justin Stoler, and Gordon A. AwandareComments to Author 
Author affiliations: University of Ghana, Legon, Ghana (N. Amoako, S. Duodu, F.E. Dennis, J.H.K. Bonney, L. Mosi, T. Hayashi, E.E. Agbosu, D. Pratt, G.E. Armah, G.A. Awandare); Kintampo Health Research Centre, Kintampo, Ghana (N. Amoako, K.P. Asante); Ledzokuku Krowor Municipal Assembly Hospital, Teshie, Accra, Ghana (J. Ameh); Tokyo Medical and Dental University, Tokyo, Japan (T. Hayashi); University of Virginia, Charlottesville, Virginia, USA (D.J. Operario, J. Liu, E.R. Houpt); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (B. Fields); University of Miami, Florida, USA (J. Stoler).

Cite This Article


We report new molecular evidence of locally acquired dengue virus infections in Ghana. We detected dengue viral RNA among children with suspected malaria by using a multipathogen real-time PCR. Subsequent sequence analysis revealed a close relationship with dengue virus serotype 2, which was implicated in a 2016 outbreak in Burkina Faso.

The accurate diagnosis of nonmalarial febrile illnesses remains a large challenge in many malaria-endemic countries (1). The etiologic agents in this context are often not identified because of nonspecific clinical symptoms and diagnostic limitations (2); for example, of 457 patients in Nigeria who were presumptively treated for malaria, only 3.9% tested positive (3). Because of the decline in malaria transmission over the past decade in many endemic areas, including Ghana, there is a critical need for a comprehensive characterization of the etiology of acute febrile illness (AFI) (4). Dengue virus infections cause symptoms that are similar to those of malaria, and considering the increasing reports of dengue outbreaks in countries neighboring Ghana (58), there is an increased need for dengue surveillance.

The Study

To obtain a description of the pathogens causing febrile illnesses in Ghana, we conducted a hospital-based cross-sectional study among children in 2 geographically distinct areas. Kintampo, in the Brong Ahafo region, is a semiurban area in the forest savanna middle belt and has a population of 42,957. Teshie is a periurban area in the Greater Accra region that has ≈171,875 residents (Technical Appendix Figure). We conducted the study during October 2016–July 2017, encompassing parts of the dry season (November–March) and rainy season (April –July). Children 1–15 years of age whose symptoms included fever were examined in the outpatient departments of Kintampo Municipal Hospital or Ledzokuku Krowor Municipal Assembly Hospital in Teshie. The study was approved by the ethics review committees of the Noguchi Memorial Institute for Medical Research, University of Ghana, the Ghana Health Service, and the Kintampo Health Research Centre. We recruited the patients for this study after we obtained written informed consent from their parents or guardians. The inclusion criteria were fever within the preceding 24 hours or measured axillary temperature ≥38°C occurring for <7 days and no severe or known chronic disease. Attending clinicians at the 2 sites screened a total of 10,234 children, and 700 were enrolled for the study on the basis of the inclusion criteria. We collected venous blood (5 mL) from each participant into EDTA-containing tubes (BD Vacutainer; Becton Dickinson, Franklin Lakes, NJ, USA) for malaria tests and full blood counts; the remaining blood was stored at −80°C until use. All of the children were treated according to the Ghana Health Service treatment guidelines.

We randomly selected stored blood samples from 166 children diagnosed with AFI and screened the samples by using a customized multipathogen, real-time PCR–based TaqMan probe-array card (TAC; Applied Biosystems, Carlsbad, CA, USA), as described by Liu et al. (9). The AFI TAC assay simultaneously tests for 26 pathogens, including 3 protozoa, 7 bacteria, and 16 viruses (Table 1). Each card tests 6 samples and 2 controls (9).

Figure 1

Thumbnail of TaqMan array card amplification plots for 2 dengue virus–positive samples and 1 negative sample in study of dengue virus among 166 children with suspected malaria, Accra, Ghana, October 2016–July 2017. Blood samples (2.5 mL of the 5.0 mL collected) obtained from children reporting to the hospital with acute febrile illness (AFI) were screened for 26 pathogens simultaneously by using the real-time PCR TaqMan array card. The cards were in 384-well format, and each well contained 1 µL

Figure 1. TaqMan array card amplification plots for 2 dengue virus–positive samples and 1 negative sample in study of dengue virus among 166 children with suspected malaria, Accra, Ghana, October 2016–July 2017. Blood...

As we expected, Plasmodium spp. was the predominant pathogen detected in samples from the children (36.8% of samples tested; Table 2). Salmonella enterica serovar Typhi, Rickettsia spp., Coxiella burnetii, and HIV-1 were also detected in some samples (Table 2). However, the most notable observation was the detection of dengue virus in samples from 2 children, 3 and 14 years of age, who were admitted to Ledzokuku Krowor Municipal Assembly Hospital; critical cycle threshold (Ct) for these children was 24.40 and 19.35, respectively (Figure 1). Furthermore, 1 of the dengue-positive samples was also positive for Plasmodium spp., providing a vivid demonstration of the complex etiology of AFI in malaria-endemic areas. This observation is consistent with a recent report showing that 51% of febrile children in Ghana who were diagnosed with malaria parasitemia were co-infected with >1 pathogen (10).

Figure 2

Thumbnail of Gel electrophoresis of dengue virus–specific RT-PCR products in study of dengue virus among 166 children with suspected malaria, Accra, Ghana, October 2016–July 2017. We completed a conventional RT-PCR assay by using dengue-specific primers from Lanciotti et al. (15) to confirm the results of the TaqMan array card assays. The amplification products (expected size 511 bp) were electrophoresed on 2% agarose gel, stained with ethidium bromide, and viewed under ultraviolet light. Lane 1

Figure 2. Gel electrophoresis of dengue virus–specific RT-PCR products in study of dengue virus among 166 children with suspected malaria, Accra, Ghana, October 2016–July 2017. We completed a conventional RT-PCR assay by using...

No dengue virus infections have previously been reported in Ghana, despite suspected transmission after the isolation of dengue virus type 2 (DENV-2) in travelers from Finland who visited Ghana during 2000–2005 (11) and our previous study showing serologic evidence of prior dengue exposure among malaria-positive children in Ghana during 2011–2014 (12). Therefore, we sought to confirm the results of the TAC screening by using 3 additional molecular methods: 1) the Trioplex TaqMan-based real-time reverse transcription PCR (RT-PCR) developed by the Centers for Disease Control and Prevention (13), which differentiates dengue from the arboviruses chikungunya (CHIKV) and Zika; 2) a second TaqMan-based real-time RT-PCR assay developed by Johnson et al. for dengue virus serotype detection (14); and 3) a conventional RT-PCR assay by using primers as described by Lanciotti et al. (15) (Figure 2). All of these methods confirmed the samples to be dengue positive. Ct values for DENV obtained from the Trioplex assay for the 2 samples were 26.96 and 34.70; results for CHIKV and Zika virus were undetermined (negative). We recorded Ct values of 27.53 and 35.42 on the Johnson et al. real-time RT-PCR assay, which also characterized the 2 samples as DENV-2.

We further sequenced viral RNA by using an ABI 3130XL Genetic Analyzer (Applied Biosystems, Carlsbad, CA, USA). BLAST search ( using default settings of generated nucleotide sequences revealed that the viruses in both positive samples were identical (100% homology) and closely related to the DENV-2 strains isolated in the 2016 outbreak in Burkina Faso (GenBank accession no. KY627763.1). We deposited the nucleotide sequences of the detected virus strain in GenBank under accession nos. MG937762 and MG937763.

Apart from fever, both dengue-positive children reported chills, cough, and vomiting; however, neither reported diarrhea or rash. On a follow-up visit 2 months after enrollment, the children from whom we obtained the dengue-positive samples seemed healthy and afebrile, suggesting that no severe complications of the infection developed. Convalescent-phase blood samples from both patients tested positive for dengue-specific IgG; 1 was additionally positive for dengue IgM (Abcam human anti-dengue virus IgG and IgM ELISA kit; Abcam, Cambridge, UK). The parents reported that these children had not traveled outside the country, indicating that the infection was locally acquired.


Our previous investigations detected dengue antibodies in 21.6% of children in 3 areas of Ghana, including Kintampo and Accra; however, no virus was detected from any of them, suggesting previous exposure rather than acute infections (12). In this study, we have now confirmed the presence of dengue virus in the blood of two children, indicating acute infections. In light of other reports from elsewhere in West Africa (57), this DENV-2 strain may soon become regionally endemic, if it has not already. We have advised the Ghana Health Service accordingly to take measures to intensify surveillance, consistent with prior recommendations (8).


Mr. Amoako is a PhD student at the University of Ghana, Legon. His research interests include investigating the etiology of acute febrile illness and identification of biomarkers of diseases.



We are grateful to the study participants for volunteering for the study and the staff of the Ledzokuku Krowor Municipal Assembly Hospital and the Kintampo Health Research Centre for their support with sample collection. We also thank Wangeci Gatei and Joel Montgomery for facilitating some aspects of the study.

This work was supported by funds from the Government of Ghana through a World Bank African Centres of Excellence grant (ACE02-WACCBIP to G.A.A.), a DELTAS Africa grant (DEL-15-007 and Wellcome Trust 107755/Z/15/Z to G.A.A.), a Royal Society Pfizer Award grant (to G.A.A.), and the Japan Agency for Medical Research and Development.



  1. Bisoffi  Z, Buonfrate  D. When fever is not malaria. Lancet Glob Health. 2013;1:e112. DOIPubMedGoogle Scholar
  2. Malm  KL, Bart-Plange  C, Armah  G, Gyapong  J, Adjei  SO, Koram  K, et al. Malaria as a cause of acute febrile illness in an urban pediatric population in Ghana. Am J Trop Med Hyg. 2012;5(Suppl 1):401.
  3. Isiguzo  C, Anyanti  J, Ujuju  C, Nwokolo  E, De La Cruz  A, Schatzkin  E, et al. Presumptive treatment of malaria from formal and informal drug vendors in Nigeria. PLoS One. 2014;9:e110361. DOIPubMedGoogle Scholar
  4. Stoler  J, Awandare  GA. Febrile illness diagnostics and the malaria-industrial complex: a socio-environmental perspective. BMC Infect Dis. 2016;16:683. DOIPubMedGoogle Scholar
  5. Oyero  OG, Ayukekbong  JA. High dengue NS1 antigenemia in febrile patients in Ibadan, Nigeria. Virus Res. 2014;191:5961. DOIPubMedGoogle Scholar
  6. Ridde  V, Agier  I, Bonnet  E, Carabali  M, Dabiré  KR, Fournet  F, et al. Presence of three dengue serotypes in Ouagadougou (Burkina Faso): research and public health implications. Infect Dis Poverty. 2016;5:23. DOIPubMedGoogle Scholar
  7. Tarnagda  Z, Cissé  A, Bicaba  BW, Diagbouga  S, Sagna  T, Ilboudo  AK, et al. Dengue Fever in Burkina Faso, 2016. Emerg Infect Dis. 2018;24:1702. DOIPubMedGoogle Scholar
  8. Stoler  J, Al Dashti  R, Anto  F, Fobil  JN, Awandare  GA. Deconstructing “malaria”: West Africa as the next front for dengue fever surveillance and control. Acta Trop. 2014;134:5865. DOIPubMedGoogle Scholar
  9. Liu  J, Ochieng  C, Wiersma  S, Ströher  U, Towner  JS, Whitmer  S, et al. Development of a TaqMan array card for acute-febrile-illness outbreak investigation and surveillance of emerging pathogens, including Ebola virus. J Clin Microbiol. 2016;54:4958. DOIPubMedGoogle Scholar
  10. Hogan  B, Eibach  D, Krumkamp  R, Sarpong  N, Dekker  D, Kreuels  B, et al. Malaria coinfections in febrile pediatric inpatients : a hospital-based study from Ghana. 2018;(March):3–10.
  11. Huhtamo  E, Uzcátegui  NY, Siikamäki  H, Saarinen  A, Piiparinen  H, Vaheri  A, et al. Molecular epidemiology of dengue virus strains from Finnish travelers. Emerg Infect Dis. 2008;14:803. DOIPubMedGoogle Scholar
  12. Stoler  J, Delimini  RK, Bonney  JHK, Oduro  AR, Owusu-Agyei  S, Fobil  JN, et al. Evidence of recent dengue exposure among malaria parasite-positive children in three urban centers in Ghana. Am J Trop Med Hyg. 2015;92:497500. DOIPubMedGoogle Scholar
  13. Centers for Disease Control and Prevention. Fact sheet for healthcare providers: interpreting Trioplex Real-time RT-PCR Assay (Trioplex rRT-PCR) results. 2017;(March).
  14. Johnson  BW, Russell  BJ, Lanciotti  RS. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. J Clin Microbiol. 2005;43:497783. DOIPubMedGoogle Scholar
  15. Lanciotti  RS, Calisher  CH, Gubler  DJ, Chang  GJ, Vorndam  AV. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol. 1992;30:54551.PubMedGoogle Scholar




Cite This Article

DOI: 10.3201/eid2408.180341

Table of Contents – Volume 24, Number 8—August 2018

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Please use the form below to submit correspondence to the authors or contact them at the following address:

Gordon A. Awandare, University of Ghana, West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, PO Box LG54, Volta Road, Legon Accra, Ghana

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Page created: July 18, 2018
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