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Volume 24, Number 9—September 2018
Research

Novel Orthopoxvirus and Lethal Disease in Cat, Italy

Gianvito Lanave, Giulia Dowgier, Nicola DecaroComments to Author , Francesco Albanese, Elisa Brogi, Antonio Parisi, Michele Losurdo, Antonio Lavazza, Vito Martella, Canio Buonavoglia, and Gabriella Elia
Author affiliations: University of Bari, Valenzano, Italy (G. Lanave, G. Dowgier, N. Decaro, M. Losurdo, V. Martella, C. Buonavoglia, G. Elia); La Vallonea Veterinary Laboratory, Milan, Italy (F. Albanese); Centro Veterinario Montarioso, Siena, Italy (E. Brogi); Istituto Zooprofilattico Sperimentale di Puglia e Basilicata, Putignano, Italy (A. Parisi); Istituto Zooprofilattico Sperimentale di Lombardia ed Emilia Romagna, Brescia, Italy (A. Lavazza)

Main Article

Table 1

PCR approach for identification of viruses of the subfamily Chordopoxvirinae*

Specificity Target gene Reference Result Amplicon, bp Sequence First match by BLAST analysis† Nucleotide identity, %
Panchordopoxvirus, low GC Insulin metalloproteinase-like protein gene/IMV membrane protein gene (19) + 220 + ECTV Naval KJ563295 100
Panchordopoxvirus, high GC Insulin metalloproteinase-like protein gene/IMV membrane protein gene (19) ND ND ND ND ND
Eurasian/African OPXVs A-type inclusion protein gene (20) + 1, 237‡ + CPXV Germany 91–3 DQ437593 98
Eurasian/African OPXVs Hemagglutinin gene (21) + 864§ + Feline poxvirus ITA2 FJ445747 96
ECTV Hemagglutinin gene (22) + 150 + ECTV Naval KJ563295 99
CPXV Hemagglutinin gene (22) ND 629–677 ND ND ND

*CPXV, cowpox virus: ECTV, ectromelia virus; IMV, intracellular mature virus; ND, not determined; OPXV, orthopoxvirus; +, sequence obtained.
†https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch. GenBank accession numbers are provided.
‡Expected size for CPXV: 1,601 or 1,673 bp; expected size for ECTV: 1,220 bp.
§Expected size for CPXV: 942 bp; expected size for ECTV: 846 bp.

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