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Volume 24, Number 9—September 2018
Dispatch

Association of Batai Virus Infection and Encephalitis in Harbor Seals, Germany, 2016

Wendy K. Jo1, Vanessa M. Pfankuche1, Annika Lehmbecker, Byron Martina, Ana Rubio-Garcia, Stefanie Becker, Jochen Kruppa, Klaus Jung, Daniela Klotz, Julia Metzger, Martin Ludlow, Wolfgang Baumgärtner, Erhard van der Vries2, and Albert OsterhausComments to Author 
Author affiliations: University of Veterinary Medicine Hannover, Hannover, Germany (W.K. Jo, V.M. Pfankuche, A. Lehmbecker, S. Becker, J. Kruppa, K. Jung, D. Klotz, J. Metzger, M. Ludlow, W. Baumgärtner, E. van der Vries, A. Osterhaus); Center for Systems Neuroscience, Hannover (W.K. Jo, V.M. Pfankuche, W. Baumgärtner, A. Osterhaus); Artemis One Health, Delft, the Netherlands (B. Martina, A. Osterhaus); Seal Centre, Pieterburen, the Netherlands (A. Rubio-Garcia)

Main Article

Figure 1

Histologic analysis and fluorescent in situ hybridization (FISH) of a Batai virus BATV)–infected harbor seal, Germany, 2016. A) Cerebellum showing mild-to-moderate, perivascularly accentuated, lymphohistiocytic inflammation (hematoxylin and eosin stain; scale bar indicates 200 μm). B) Purkinje cells and neurons of granular cell layer showing intracytoplasmic BATV–specific pink, positive result detected by FISH (fast red stain; scale bar indicates 200 µm). Inset: Higher magnification view of anal

Figure 1. Histologic analysis and fluorescent in situ hybridization (FISH) of a Batai virus BATV)–infected harbor seal, Germany, 2016. A) Cerebellum showing mild to moderate, perivascularly accentuated, lymphohistiocytic inflammation (hematoxylin and eosin stain; scale bar indicates 200 μm). B) Purkinje cells and neurons of granular cell layer showing intracytoplasmic BATV–specific pink, positive result detected by FISH (fast red stain; scale bar indicates 200 µm). Inset: Higher magnification view of analysis using the QuantiGene ViewRNA ISH Tissue 1-Plex Assay Kit and the QuantiGene ViewRNA Chromogenic Signal Amplification Kit (Affymetrix-Panomics, Santa Clara, CA, USA) (fast red stain; scale bar indicates 20 µm). C) Scattered neurons of spinal cord showing a strong, pink, intracytoplasmic BATV-specific result detected by FISH (fast red stain; scale bar indicates 100 µm). D) Cortical and medullary lymphocytes of pulmonary lymph node showing a mild, pink, intracytoplasmic BATV-specific result detected by FISH (fast red stain; scale bar indicates 20 µm). E). Negative control (incubation without probe) of spinal cord showing no BATV-specific result (fast red stain; scale bar indicates 100 µm).

Main Article

1These authors contributed equally to this article.

2Current affiliation: Utrecht University, Utrecht, the Netherlands.

Page created: August 15, 2018
Page updated: August 15, 2018
Page reviewed: August 15, 2018
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